血管紧张素转换酶基因多态性及其酶活性与冠心病关系的研究  

Relationship Between Angiotensin Converting Enzyme Gene Polymorphism,Angiotensin Converting Enzyme Activity And Coronary Heart Disease

作　　者：刘华欣[1] 曹丽春[1] 廖淑梅[2] 王吉伟[2] 傅桂莲[3] 

机构地区：[1]第四军医大学吉林军医学院,吉林132013 [2]中南大学湘雅医学院 [3]北华大学医学院中心实验室

出　　处：《中国医师杂志》2002年第5期483-485,共3页Journal of Chinese Physician

摘　　要：目的　研究血管紧张素转换酶 (ACE)基因多态性、血管紧张素转换酶活性与冠心病的相关关系。方法　应用聚合酶链式反应 (PCR)检测ACE基因第 16内含子I/D多态性 ,并计算基因型和等位基因频率。应用酶偶联速率法检测血清血管紧张素转换酶活性。结果　在 5 1例冠心组中ACE基因DD基因型和D等位基因频率分别是 35 %和 6 1% ,ACE平均活性为 35 0 3± 91 1,在 83例正常对照组中的ACE基因DD基因型和D等位基因频率分别是 16 %和 4 5 % ,ACE平均活性为 2 86 7± 79 6 ,两者相比具有显著性差异。结论　ACED等位基因可能是冠心病潜在的危险因素之一 。Objective The relationship among angiotensin converting enzyme(ACE) gene polymorphism,angiotensin converting enzyme activity and coronary heart disease(CHD) was studied.Method Teh polymerase chain reaction was used to detect an insertion/deletion(I/D) of ACE gene polymorphism in the sixteen intron,then genotype and allele freqencies were counted .The method of enzyme coupling ratio was used to detect ACE activity .Results In 51 case with CHD ,the DD genotype and D allele freqencies of ACE gene were 35% and 61% respectively ,ACE average activity was (350 3±91 1)su/L.In 83 normal control subject,they were 16% and 45% and (286 7±79 6)su/L respectively.The freqencies of DD genotype and D allele and ACE average activity had significant difference.Conclusion The deletion polymorphism of ACE gene might be an latent risk factor.The higher ACE activity might play an important role in the development of CHD.

关 键 词：血管紧张素转换酶 基因多态性 冠心病 酶活性 聚合酶链式反应 

分 类 号：R541.4[医药卫生—心血管疾病]