CBN中有效成分葛根素在大鼠体内的排泄  

Excretion of puerarin from the rats after CBN injection

作　　者：王莉[1] 邢东明[2] 卢弘[2] 孙虹[2] 何希辉[2] 杜力军[2] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094 [2]清华大学生物科学技术系药物药理研究室,北京100084

出　　处：《中药药理与临床》2001年第5期8-10,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：科技部 95攻关项目合同号 :96 - 90 1 - 0 5 - 1 0 5

摘　　要：目的 :研究CBN注射剂中葛根素在大鼠体内排泄的特点。方法 :取大鼠尾静脉注射 10 0mg/kg给药后不同时间分别收集尿和粪便。乌拉坦麻醉大鼠做胆总管插管 ,于股静脉给药后不同时间采集胆汁。以HPLC外标法测定样品中葛根素含量 ,计算不同时间自尿粪胆汁排出量及占给药量百分比。结果 :静脉给药后 12h药物自尿排出给药量的 34 6 % ,其中 85 6 %在前 2h从尿中排出 ,12h后尿中几无法测出。粪便中葛根素含量很少 ,胆汁中葛根素在药后 8h时累积排泄量为 12 7μg/ml ,占总排泄量的2 7% ,其中 70 4%于前 2h内排出。结论 :静脉注射CBN后其葛根素原型主要以尿中排出 ,而排出时间集中在药后的前 2h。Study of the typical discharge of puerarine from the rat after iv CBN injection. Methods: After iv CBN injection, collecting the urine, stools and bile from the rat at different times. Testing the concentration of purarine by the way of HPLC, extrostandered, after preparing the tested sample. Analyzing the date, to determine the excreted ratio of both urine and stool. Results: After 12 hours on CBN injection, 34.6% puerarin was disccharged by urine, among it, 85.6% puerarin discharged at the first 2 hours. It can not detected after 12 hours. There is few puerarine can be test in the stool. The accumulation of puerarin in bile is 12.7μg/ml after CBN injected 8 hours, which is 2.7% of total excretion of puerarin, among which there was 70.4% of puerarin excretion in the first 2 hours after injection. Conclusion: Puerarine in CBN injection was mainly discharged by urine.

关 键 词：CBN 葛根素 大鼠 排泄 

分 类 号：R285.5[医药卫生—中药学]