不同梅毒螺旋体基因重组抗原酶联免疫试验比较  

Comparison of Different Recombinant Antigen-Based Enzyme Immunoassays for Detection of Antibodies Against Treponema Pallidum

作　　者：张瑞芬[1] 庄辉[1] 蒋志彬[2] 王薇媛[2] 杜兰英[1] 刘学恩[1] 

机构地区：[1]北京大学基础医学院微生物学教研室,100083 [2]中国药品生物制品检定所

出　　处：《中国公共卫生》2002年第6期673-674,共2页Chinese Journal of Public Health

摘　　要：目的　对单一梅毒螺旋体 (Treponemapallidum ,Tp)基因重组抗原Tp47、Tp42或Tp1 7及联合应用 3种基因重组抗原 (Tp47、Tp42和Tp1 7)酶联免疫试验 (enzymeimmunoassay,EIA)进行比较。 方法　分别用 3种单一Tp基因重组抗原Tp47、Tp42或Tp1 7EIA法 ,检测 1 1份梅毒血凝试验Tp抗体阳性和 2 8份阴性血清 ,并与联合应用 3种基因重组抗原 (Tp47、Tp42和Tp1 7)EIA法比较。 结果　单一基因重组抗原Tp47、Tp42或Tp1 7EIA法的灵敏度分别为1 0 0 % (1 1 / 1 1 )、1 0 0 % (1 1 / 1 1 )和 90 9% (1 0 / 1 1 ) ,特异度分别为 96 4% (2 7/ 2 8)、1 0 0 % (2 8/ 2 8)和 96 4% (2 7/ 2 8) ;联合应用 3种基因重组抗原EIA法的灵敏度和特异度分别为 1 0 0 % (1 1 / 1 1 )和 1 0 0 % (2 8/ 2 8)。结论　单一Tp基因重组抗原EIA法用于梅毒诊断存在一定的假阳性和假阴性反应 ,联合应用 3种基因重组抗原 (Tp47、Tp42和Tp1 7)Objective To compare the enzyme immunoassays (EIA) based on a single recombinant Treponema pallidum (TP) antigen Tp47,Tp42 or Tp17 with that using combination of the three recombinant Tp antigens.Methods Anti-Tp negaove and 10 anti-Tp positive sera tested by TPHA (Treponema pallidum hemagglutination) were detected by both the EIA based on combination of the 3 recombinant Tp antigens (Tp47,Tp42 and Tp17) and the EIA using a single Tp protein (Tp47,or Tp42,or Tp17).Results The sensitivity of EIA based on a single antigen Tp47,Tp42,or Tp17 was 100% (11/11),100% (11/11) and 90.9%(10/11),with the speciacity of 96 4% (27/28),100% (28/28) and 96 4% (27/28),respectively.The sensitivity and the specificity of EIA based on combination of the three Tp antigens were 100% (11/11) and 100% (28/28),respectively.Conclusion There was some false negative and false positive reactivity with the EIA based on a single recombinant antigen for diagnosis of syphilis.The sensitivity of EIA based on combination of the three antigens (Tp47,Tp42 and Tp17) for detection of anti-Tp antibody was higher than that of the EIA with a single recombinant Tp antigen.

关 键 词：梅毒螺旋体 梅毒 基因重组抗原 诊断 酶联免疫试验 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]