HBcAg显性失活突变体质粒的构建及体外表达  

The Construction of Dominant Negative Mutant Plasmid pCDNA4 - C - GFPand Its in vitro Expression

作　　者：吴大勇[1] 周霞秋[1] 王斌[1] 俞红[1] 郭清[1] 徐人欢[1] 郭强苏[2] 

机构地区：[1]上海第二医科大学瑞金医院传染科,上海200025 [2]上海第二医科大学瑞金医院组胚教研室,上海200025

出　　处：《上海第二医科大学学报》2002年第3期218-220,共3页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：目的探讨HBcAg显性失活突变体质粒pCDA4-C-GFP的构建及体外表达。方法 选择编码乙肝核心抗原C基因片段及绿色荧光蛋白（green fluorescent protein,GFP）基因片段,利用基因重组技术构建成DNA质粒pCDNA4-C-GFP,并将该质粒转染肝癌细胞株HepG2。结果 通过RT-PCR检测到其RNA的表达,Confocal观察到GFP绿色荧光。结论 HBcAg显性失活突变体质粒pCDNA4-C-GFP的构建成功可以进行对HBV作用的研究。To investigate the construction of pCDNA4 - C - GFP and its in vitro expression. Methods The DNA plasmid pCDNA4 - C- GFP, which contains the C gene encoding core protein of HBV and green fluorescent protein gene, was constructed by gene technology. The plasmid pCDNA4 - C - GFP was transferred into the cell line HepG2. The in vitro expression of the plasmid was observed. Results Transcripts of the plasmid were detected by RT- PCR, and the GFP expession was detected by confocal. Conclusion The plasmid pCDNA4 - C- GFP can be used for its functional research.

关 键 词：乙肝核心抗原 显性失活突变体 绿色荧光蛋白 质检构建 体外表达 

分 类 号：R392[医药卫生—免疫学]