大鼠中枢5-HT_(1A)受体的结合容量与高血压  

Binding capacity of 5-HT_(1A) receptor in central nervous system and hypertension in rats

作　　者：周静[1] 唐仲进[2] 刘垣升[2] 苏定冯[2] 刘皋林[1] 

机构地区：[1]第二军医大学长征医院药学部,上海200003 [2]第二军医大学基础医学部药理学教研室

出　　处：《第二军医大学学报》2002年第5期523-525,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目 (3 0 170 3 85 )

摘　　要：目的 :探讨 5 - HT1 A受体结合容量改变对高血压发病的影响。方法 :通过放射性配体与受体结合实验 ,探讨不同周龄自发性高血压大鼠 (SHR)和对照组 Wistar- Kyoto (WKY)大鼠在脑区不同部位 5 - HT1 A受体的结合特征。 结果 :SHR不同脑区脑组织膜的 Bmax随着大鼠周龄的增加而增大 ,且有显著差异 ;WKY鼠差异不明显。相同脑区不同周龄 SHR的 Bmax均大于WKY大鼠 ,且随着周龄的增加血压呈上升趋势 ,其 5 - HT1 A受体的 Bmax与血压成正比 ,WKY无此现象。 4～ 5周龄的 SHR为高血压未形成期 ,10～ 12及以上周龄的 SHR处于高血压的形成期。结论 :当血压发生变化时 ,中枢 5 - HT1 A受体结合容量发生继发性改变。不同脑区中枢 5 - HT1Objective: To study the relationship between the 5 HT 1A receptor specific binding in central nervous system(CNS) and hypertensive stage in rats. Methods: The specific binding of 8 OH DPAT to 5 HT 1A receptor in related sites was investigated in the membrane preparation of several brain regions. Results:Significant differences in the B max and K d were observed in the membrane preparation of medulla oblongata and hypothalamus. WKY 8 OH DPAT binding site were significantly increased compared with that of SHR with the development of hypertension. K d of 4 5 weeks,10 12 weeks,20 22 weeks and >40 weeks old SHR did not differ from that in slices of age matched WKY. B max was increased in the same brain region of SHR when compared to WKY. That rats of 4 5 weeks were in prehypertensive stage;rats of 10 12 weeks and above were at the stage of establishing hypertensive stage. Conclusion: The difference between SHR and normotensive rats in 5 HT 1A receptor binding in various brain regions may be related to the development of hypertension. When blood pressure changes,binding capacity of 5 HT 1A receptor in CNS changes accordingly.

关 键 词：大鼠 5-HT1A受体 结合容量 高血压 脑区中枢 

分 类 号：R544.1[医药卫生—心血管疾病]