三七中人参三醇苷对脑缺血的保护作用及其机制  

Protective Effects and its Mechanism of Panaxatriol Saponins Isolated from Panax notoginseng on Cerebral Ischemia

作　　者：姚小皓[1] 李学军[1] 

机构地区：[1]北京大学基础医学院药理学系,北京100083

出　　处：《中国中药杂志》2002年第5期371-373,共3页China Journal of Chinese Materia Medica

摘　　要：目的 :研究三七中人参三醇苷 (PTS)对大鼠局灶性脑缺血的保护作用及作用机制。方法 :以大鼠永久性大脑中动脉阻塞模型 (MCAO)为基础 ,采用免疫转移印迹 (WesternBlot)的方法 ,观察PTS对大鼠脑缺血的保护作用及 3种脑缺血相关蛋白 (VEGF ,HSP70和转铁蛋白 )表达的影响。结果 :ipPTS 12 .5mg·kg-1× 7d(术前 5d ,术后 2d)可降低MCAO大鼠脑含水量 ,并影响两种脑缺血相关蛋白 (HSP70和转铁蛋白 )的含量 ,但对VEGF表达量在手术后的变化没有明显的影响。结论 :PTS对大鼠局灶性脑缺血有一定的保护作用 ,其机制可能是上调HSP70和下调转铁蛋白 。Objective: To study the protective effects and its mechanism of Panaxatriol Saponins isolated from Panax notoginseng (PTS) on focal cerebral ischemia in rat brain. Method: The influences of PTS on cerebral water content and three specific proteins (VEGF, HSP70 and transferrin) related with cerebral ischemia were studied with unilateral occlusion of the middle cerebral artery (MCAO) and Western Blot. Result: PTS 12.5 mg·kg -1 ip×7 d (5 d before MCAO and 2 d after MCAO) inhibited the increase of cerebral water content caused by MCAO and influenced contents of HSP70 and transferrin, but had no influence on VEGF protein level. Conclusion: PTS shows a protective effect on focal cerebral ischemia in rat brain by alleviating cerebral edema, up regulating the expression of HSP70, down regulating transferrin and maintaining blood brain barrier.

关 键 词：脑缺血 三七 人参三醇苷 大脑中动脉阻塞 脑含水量 免疫印迹 药理学 

分 类 号：R285.5[医药卫生—中药学]