胰腺内分泌A细胞与胆碱能神经关系的探讨——迷走神经背核VAChT免疫组化研究  

The Study of the Relationship between Endocrinal Islet A Cell and Cholinergic Neurons: VAChT Immunohistochemistry Study on Dorsal Motor Nucleus of the Vagus Nerve in Selective Injured Islet A Cell of Rat

作　　者：张晓杰[1] 兴桂华[1] 刘婷[1] 梅双[1] 李织[1] 

机构地区：[1]齐齐哈尔医学院病理教研室,黑龙江齐齐哈尔161042

出　　处：《解剖科学进展》2002年第2期113-114,共2页Progress of Anatomical Sciences

摘　　要：目的　探讨胰腺内分泌A细胞与胆碱能神经的关系。方法　选用硝酸钴选择性破坏大鼠胰腺A细胞 ,并用特异性较强的VAChT抗血清 ,从形态学上观察模型大鼠胆碱能神经在延脑迷走神经背核的变化。结果　模型大鼠VAChT免疫反应明显减弱。结论　内源性乙酰胆碱的变化与胰腺A细胞损伤有关 ,提示胆碱能神经除受其上级中枢调节外 。Objective To study the relationship between endocrinal islet A cell and cholinergic neurons. Methods We selected Co(NH 3) 2·6H 2O to destroy islet A cell selectively,and utilized very peculiar VAChT antibody to observe the changes of cholinergic neurons in dorsal motor nucleus of the vagus nerve (DMV) in model rats. Results VAChT immunohistochemistry reaction was decreased obviously in Co(NH 3) 2·6H 2O treated group compared with the normal control group. Conclusion The change of acetylcholine has relation to injury of islet A cell .This phenomenon indicates that cholinergic neuron has been controlled and regulated by the higher level centres,and by some endocrine cells too.

关 键 词：VACHT 胰岛A细胞 胆碱能神经 迷走神经背核 小泡乙酰胆硷转运体 免疫组织化学 胰腺 

分 类 号：R329.3[医药卫生—人体解剖和组织胚胎学]