全厚皮片移植后表皮黑素细胞形态学变化  

Morphological changes of melanocytes in the epidermis of skin autografts

作　　者：李海洋[1] 李武修[1] 王丽芳[1] 

机构地区：[1]滨州医学院附属医院口腔颌面外科,256603

出　　处：《中华医学美学美容杂志》2002年第3期121-124,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：山东省自然科学基金资助项目 (92C0 72 4)

摘　　要：目的　研究黑素细胞 (MC)形态与功能的关系及其对全厚皮片移植后色泽变化的可能影响。方法　黑白花豚鼠 18只 ,随机分为 9组 ,每组 2只 ,分别于皮片移植术前 (对照组 )及术后 1、2、3、7、14、2 1、2 8、90d取材。使用表皮铺片及多巴反应技术 ,显示不同时间点皮片表皮MC ;然后借助电子计算机辅助图像分析系统对MC的形态学变化进行观察及量化分析。结果　皮片移植术后 3d内 ,手术损伤及缺血缺氧导致MC突起脱落 ,MC总面积、突起面积及突起数目减少 ;术后 2～ 3周时各项指标恢复至术前水平。实验末期 ,特征性的表现是MC突起粗而短。结论　全厚皮片内MC的形态学变化与其功能变化基本一致 ;与MC胞体形态及面积变化相比较 ,其突起的数目、面积变化能更敏感地反映细胞的功能状态 ;与移植前正常状态下MC细而长的突起相比 ,术后 1～ 3个月MC短而粗的突起适应了皮片移植后期较小的表皮黑素单位 (EMU)的需要 。Objective To study the relationship between morphological and functional changes of melanocytes and their effect on the graft color. Methods Eighteen black whited guinea pigs were divided into nine groups randomly and biopsied at 1 d, 2 d, 3 d, 7 d, 14 d, 21 d, 28 d, and 90d after skin grafting, respectively. Then skin spliting and Dopa reaction techniques were used to demonstrate melanocytes. And computer assitant imagine analysis system was applied to observe and quantitate the morphological changes of melanocytes at various time points in three postoperative months. Results During three days after grafting, resulting from the surgery wound and shortage of blood and oxygen supply, the processes of the melanocytes broke off, accompanied by reduction of whole dimension of the cells, size as well as number of the processes. The indices above returned to the normal level between second week and third week after grafting. It was an evident characteristic that number of melanocytic processes became shorter and stouter in the end of the experiment. conclusion Functional changes of melanocytes in the skin grafts are in accord with their morphological changes. Compared to those in the sizes of melanocytes, changes in the processes of the cell are more sensitive in reflection of function of melanocytes. The shorter and stouter processes of melanocytes in the end of the experiment are suitable to the smaller epidermal melanin unit and more efficient in transformation of melanin into the keratinocytes.

关 键 词：全厚皮片移植 表皮 形态学 皮肤移植 黑素细胞 

分 类 号：R622[医药卫生—整形外科]