瘢痕增生患者T淋巴细胞的变化  

Changes of cellular immunity in patients with hypertrophic sear

作　　者：王锡华[1] 吴军[2] 谷才之[1] 周立新[2] 张宁[2] 肖光夏[2] 

机构地区：[1]兰州军区乌鲁木齐总医院烧伤整形科,830000 [2]第三军医大学西南医院烧伤研究所

出　　处：《中华医学美学美容杂志》2002年第3期149-151,共3页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的　探讨瘢痕增生患者T淋巴细胞免疫功能有无改变。方法　对 6例瘢痕疙瘩、14例增生性瘢痕患者和 10例正常人外周血淋巴细胞 ,用流式细胞仪进行T淋巴细胞及其亚群分析测定 ,并在体外淋巴细胞培养中用刀豆素A(ConA)刺激淋巴细胞增殖 ,用 3H TdR掺入法测定淋巴细胞增殖活性。结果　瘢痕疙瘩组的外周血淋巴细胞中CD3+ 、CD4 + 、CD8+ 、CD4 + CD8+ 及淋巴细胞刺激增殖指数分别为 78.6 %、4 9.7%、2 3.5 %、2 .4和 17.4。增生性瘢痕组分别为 6 8.9%、4 7.6 %、2 6 .6 %、2 .2和15 .9。而正常对照组为 6 9.2 %、37.2 %、30 .9%、1.9和 8.7。瘢痕疙瘩组与增生性瘢痕组的CD4 + 、CD4 + CD8+ 及淋巴细胞增殖活性均高于正常对照组 (P <0 .0 5 )。结论　T淋巴细胞免疫功能异常可能在瘢痕增生中起重要作用。Objective To analyse the changes of T lymphocytes and its subset in patients with scar hypertrophy. Methods In 6 keloid, 14 hypertrophic scar patients and 10 normal control, peripheral blood T lymphocytes (PBTL) and its subset were analysed with flow cytometry (FCM). Lymphocytic proliferation responsed to Con A stimulation in vitro lymphocytic culture system were performed and lymphocytic proliferation activity were assayed by the measurement of 3H TdR incorporation. Results The percent of CD3 +,CD4 +,CD8 + and ratio of CD4 +/CD8 + in PBTL of keloid patients were 78.6 %, 49.7 %, 23.5 % and 2.4, and in hypertrophic scar patients group were 68.9 %, 47.6 %, 26.6 % and 2.2, in normal control group were 69.2 %, 37.2 %, 30.9 % and 1.9, respectively. Lymphocytic proliferation activity expressed as proliferation stimulation index were 17.4,15.9 and 8.7 respectively in keloid, hypertrophic scar and normal group. The level of CD4 +?CD4 +/CD8 + and lymphocytic proliferation stimulative index were all significantly higher in both keloid and hypertrophic scar patients than that in normal control. Conclusion Aberrant immunologic function may play an important role in the pathogenesis of scar.

关 键 词：瘢痕 T淋巴细胞 变态反应 免疫学 瘢痕疙瘩 增生性瘢痕 

分 类 号：R622[医药卫生—整形外科]