一株与大鼠肝细胞再生相关的新cDNA克隆  

A novel cDNA clone related with rat liver regeneration

作　　者：汪思应[1] 程洁[1] 郑红[1] 张平[1] 张宝霆[1] 许望翔[2] 魏汉东[2] 杨晓明[2] 

机构地区：[1]安徽医科大学病理生理教研室,安徽合肥230032 [2]北京军事医学科学院放射医学研究所,北京100056

出　　处：《中国病理生理杂志》2002年第6期602-606,共5页Chinese Journal of Pathophysiology

基　　金：安徽省自然科学基金资助项目 (No .0 0 0 4 4 2 0 2 );国家自然科学基金资助 (No.396 70 36 6 )

摘　　要：目的 :用表达性差异显示分析 (RDA)技术发现的一株新基因为目的基因。研究该基因与肝细胞再生的可能关系及在组织中的分布等初步的生物学功能。方法 :用狭线杂交、Northern杂交、RT -PCR、cDNA文库筛选及序列分析法研究新基因cDNA序列、组织分布及初步功能。结果 :从大鼠胎肝cDNA文库中筛选出该基因的克隆 ;杂交等证实其在肝大部切除后表达迅速上调 ,在EGF诱导的肝细胞及肿瘤组织中呈高丰度表达 ;对该基因进行序列测定并获得全长核苷酸序列 ;分析表明该核苷酸序列仅编码 70余个氨基酸。结论 :该基因与肝细胞再生相关 ;可能仅编码小分子多肽或其N -端具有更长的序列。AIM: To study a novel gene that probably related with liver regeneration, which was found by representational difference analysis(RDA). METHODS: cDNA sequence, tissue distribution and functions of the novel gene were studied by slot blot, Northern blot, RT-PCR, cDNA library screening and sequence analyzing. RESULTS: Two full-length clones were isolated from cDNA library of rat fetal livers and the sequence analysis identified that the positive cDNA encoded 76 amino acids only; Using the cDNA as a probe, the novel gene showed a specific liver distribution, a moment increasing expression in one hour after partial hepatectomy (PH) and high expression in fetal liver or liver tumor by Northern blot; EGF quickly induced its high expression in primary culture rat hepatocytes(FCS free).CONCLUSION: These results show that the novel gene is an early phase response gene that is closely related to a liver regeneration adjustment. It may encode peptide or has longer sequence at N tip.

关 键 词：CDNA克隆 肝细胞 表达性差异显示分析 肝部分切除 肝再生 细胞增殖 

分 类 号：R657.3[医药卫生—外科学]