氧化型胆固醇下调血管平滑肌细胞金属蛋白酶组织抑制剂基因表达  

Oxysterols downregulate tissue inhibitor of metalloproteinase-1expression in vascular smooth muscle cells

作　　者：崔鸣[1] 陈凤荣[1] 宋清华[2] 朱应葆[2] 

机构地区：[1]北京大学第三医院心内科,北京100083 [2]北京大学细胞与分子生物学实验室,北京100083

出　　处：《中国病理生理杂志》2002年第6期622-624,I002,共4页Chinese Journal of Pathophysiology

基　　金：国家教委博士点科研基金资助项目 (No.972 4 )

摘　　要：目的 :研究氧化型胆固醇对血管平滑肌细胞MMP - 9及TIMP - 1表达的影响。方法 :离体培养兔主动脉血管平滑肌细胞 ,分别用胆固醇、Triol与 2 5 -OH负载细胞 ,狭缝杂交测定TIMP - 1mRNA表达 ,细胞免疫化学测定MMP - 9与TIMP - 1蛋白表达。结果 :Triol与 2 5 -OH(1mg/L ,2 4h)抑制TIMP - 1mRNA及蛋白表达 ,对MMP - 9蛋白表达无影响。结论 :氧化型胆固醇可以下调血管平滑肌细胞TIMP - 1基因表达。AIM: To investigate the effects of oxysterols on matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase-1 (TIMP-1) expression in vascular smooth muscle cells. METHODS: Rabbit aortic vascular smooth muscle cells were cultured in vitro and incubated with cholesterol, Triol and 25-hydroxycholesterol (25-OH), respectively. Slot blot was used to detect the mRNA expression level of TIMP-1 in vascular smooth muscle cells (VSMCs); meanwhile the protein expression level of MMP-9 and TIMP-1 was detected by immunohistology. RESULTS: Triol and 25-OH inhibited the expression of TIMP-1 compared with control and cholesterol, but have no effect on expression of MMP-9. CONCLUSION: Both Triol and 25-OH downregulated TIMP-1 expression in VSMCs .

关 键 词：氧化型胆固醇 血管平滑肌 金属蛋白酶类 基因表达 金属蛋白酶组织抑制剂 动脉粥样硬化 AS 发病机制 

分 类 号：R543.5[医药卫生—心血管疾病]