调控中性白细胞凋亡有关因素的体外实验  

Factors involved in regulation of polymorphonuclear neutrophils apoptosis in vitro

作　　者：刘韧[1] 田昆仑[1] 肖南[1] 刁有芳[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所,重庆400042

出　　处：《中国病理生理杂志》2002年第6期653-657,共5页Chinese Journal of Pathophysiology

摘　　要：目的 :用体外实验观察多种因素 (包括亚砷酸钠、TNF -α、IL - 6及烧伤血清、创伤血清 )对中性白细胞 (PMN)凋亡的影响 ,为探索一种新的保护组织免遭继发性损害的方法提供依据。方法 :分离纯化人外周血PMN ,与亚砷酸钠 (Ars)、TNF -α、IL - 6及烧伤血清、创伤血清作用后 ,测定凋亡比例、CD 11b表达、呼吸爆发、胞质Ca2 + 浓度的改变。结果 :Ars浓度与PMN凋亡呈剂量依赖关系 ,激活的PMN却对Ars失敏 ;IL - 6使PMN凋亡延迟 (与 2 4h对照相比 ,P <0 0 5 ) ,抑制CD 11b表达 (与 2 4h对照相比 ,P <0 0 1) ;烧伤血清和创伤血清的作用较IL - 6更为明显。结论 :发现PMN激活后对Ars失敏 ,其机理不清 ;CD 11b表达升高与PMN凋亡增多呈正相关 ;IL - 6、烧伤血清、创伤血清能延迟PMN凋亡 ,部分恢复PMN的功能。AIM: This study aimed at elucidated the possibility that prevent tissue from secondary injury by regulating polymorphonuclear neutrophil (PMN) apoptosis in vitro . METHODS:Neutrophils, isolated from peripheral blood, were incubated with sodium arsenite (Ars), tumor necrosis factor (TNF-α), interleukin-6 (IL-6), burned serum and traumatic serum, respectively. Apoptosis rate, expression of CD11b, respiratory burst and concentration of Ca 2+ were then measured. RESULTS:The elevation of PMN apoptosis rate was Ars concentration dependent, but activated PMN became insensitive to Ars. IL-6 delayed PMN apoptosis (compared with control at 24 h, P <0.05), inhibited CD11b expression. Burned and traumatic serum had more significant effects on PMN compared with IL-6. CONCLUSION:PMN was observed for the first time to resist spontaneous apoptosis when activated by LPS/TNF, and became insensitive to apoptosis-inducing substance Ars. IL-6, burned serum and traumatic serum could delay PMN apoptosis and recover PMN functions partly.

关 键 词：中性白细胞 肿瘤坏死因子 白细胞介素6 细胞凋亡 体外实验 

分 类 号：R331.142[医药卫生—人体生理学] R329.25[医药卫生—基础医学]