脐血中TCRVβ亚家族T细胞的分布和克隆性分析  

Analysis of the distribution and clonality of TCR VβT cells in cord blood

作　　者：陈少华[1] 李扬秋[1] 杨力建[1] 张玉平[1] 黎清[2] 

机构地区：[1]暨南大学医学院血液病研究所,广东广州510632 [2]暨南大学医学院第一附属医院妇产科,广东广州510632

出　　处：《中国病理生理杂志》2002年第6期662-665,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :了解正常脐血中TCRVβ亚家族T细胞的分布和克隆性。 方法 :利用RT -PCR分别扩增 13例正常脐血单个核细胞的TCRVβ 2 4个亚家族基因的CDR3,了解各Vβ亚家族的表达情况。阳性的PCR产物进一步经荧光素标记和基因扫描分析产物的CDR3长度 ,了解T细胞克隆性。 10例正常人外周血和T细胞株Molt- 4及Jurkat作为对照。结果 :正常脐血T细胞仅选择性表达 2 4个Vβ亚家族的 38 78%± 16 2 6 % ,以Vβ 3、5、8、9和 13为多见 ,而正常人外周血T细胞则表达全部 2 4个Vβ亚家族。基因扫描显示正常脐血和正常人外周血的全部PCR产物均呈多峰图象。结论 :正常脐血存在不完全TCRVβ亚家族T细胞 。AIM:To investigate the distribution and clonality of TCR Vβ subfamily T cells in cord blood. METHODS: The CDR3 of TCR Vβ 24 subfamily genes were amplified in mononuclear cells from 13 cases of cord blood. To observe the usage of TCR Vβ repertoire, the PCR products were further labeled with fluorescent and analyzed by genescan technique for the CDR3 size, to evaluate clonality of the detectable TCR Vβ T cells. Peripheral bloods from 10 cases of normal individuals and T cell line Molt-4 and Jurkat served as controls. RESULTS: Only 38.78%±16 26% of 24 Vβ subfamily T cell were selectively expressed in cord blood, predominantly in Vβ 3, 5, 8, 9 and 13, whereas all 24 Vβ subfamilies could be detected in T cells from peripheral blood of normal individuals. Genescan analysis showed that all PCR products of TCR Vβ subfamilies from cord blood or normal individual peripheral blood displayed multi-peaks. CONCLUSION: Some TCR Vβ subfamily T cells were absent in cord blood. All TCR Vβ subfamily T cells in cord blood displayed polyclonality.

关 键 词：脐血 TCR Vβ亚家族T细胞 RT-PCR方法 基因扫描分析 抗宿主病 T细胞受体 造血干细胞移植 

分 类 号：R457.7[医药卫生—治疗学]