苦瓜性别分化程序表达的研究(英文)  

Studies on expression of sex differentiation program in Momordica charantia L.

作　　者：汪俏梅[1] 曾广文[1] 

机构地区：[1]浙江大学园艺系,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2002年第3期243-248,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：Funds granted by Natural ScienceFoundation of China (30 0 0 0 15 ) ;Zhejiang Provincial Natu-ral Science Foundation (396 2 79)

摘　　要：苦瓜的性别分化过程要先经过一个两性期 ,再分别向雄性或雌性的方向发育 .对两性期和雄 ,雌花早期发育的 3个典型时期的花蕾进行毛细管电泳分析 ,结果表明 ,一种分子量为 11k D的蛋白质在雌花发育的 3个时期都存在 ,并且含量变化很小 ,很可能是雌花程序表达中的一种“关键蛋白”;类似地 ,一种分子量为 30 k D的蛋白质很可能是雄花程序表达中的一种“关键蛋白”。此外 ,蛋白质合成抑制剂亚胺环己酮抑制离体两性花的体外性别分化 ,而核酸合成抑制剂The sex differentiation process of Momordica charantia first underwent a hermaphroditic stage, then developed forward maleness or femaleness. The soluble protein profile of flower buds at hermaphroditic stage and three early typical development stages of male and female flowers were analyzed with capillary electrophoresis. The result suggested that a 11 kD protein, which existed in all the three development stages of the female flowers with little change of content, might be an 'essential protein' for the expression of female flower differentiation program. Similarly, a 30 kD protein might be an 'essential protein' for the expression of male flower differentiation program. In addition, the sex differentiation of hermaphroditic flower buds in vitro was inhibited by cycloheximide-an inhibitor of protein synthesis, but not by cordycepin a nucleic acid synthesis inhibitor.

关 键 词：苦瓜 性别分化程序 表达 特异蛋白质 早期发育 

分 类 号：Q945.6[生物学—植物学] S642.5[农业科学—蔬菜学]