石斛菌根真菌液培生长特性的研究  

Investigation on Growth Characteristics of Dendrobium 's Mycorrhizal Fungi in Liquid-culture

作　　者：陈连庆[1] 裴致达[1] 韩宁林[1] 张守英[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《林业科学研究》2002年第2期207-211,共5页Forest Research

基　　金：国家自然科学基金项目"石斛共生真菌生物学基础研究"(39870 6 2 2 ) ;浙江省自然科学基金项目 (3970 30 )

摘　　要：石斛菌根真菌液培生长特性研究结果表明 :F990 3菌株最适增殖生长温度是 30℃ ,其菌丝体平均每天生长速度最快 ,达 1 2 2 2cm ;培养液pH 3～ 8范围内F990 3菌株均能生长 ,以pH值 7时菌丝体增殖效果最好 ,干菌丝质量 1 4 5 0mg·mL- 1,增殖 131 8倍 ;提供的 8种N源 (硫酸铵、硝酸铵、硝酸钙、硝酸钾、脲、酒石酸铵、甘氨酸、谷氨酸 )中 ,吸收利用效果最好的是酒石酸铵N源 ,菌丝体干质量1 6 84mg·mL- 1,增殖 15 3倍 ,谷氨酸次之 ;提供的 8种C源 (葡萄糖、果糖、蔗糖、麦芽糖、乙醇、甘露醇、糊精、可溶性淀粉 ) ,F990 3菌株均能吸收利用 ,尤以果糖C源增殖效果最高 ,菌丝体干质量 1 5 0 1mg·mL- 1,增殖 136 5倍 ;培养液中不同含量的速效P对F990 3菌株菌丝体增殖效果 ,以处理 3(磷酸二氢钾 1mg·mL- 1)最佳 ,菌丝体干质量 1 2 5 6mg·mL- 1,增殖达 114The investigation shows that the fungus No.F9903 is much better than others. It grew faster, 1.222 cm per day under the most suitable temperature,30 ℃.Although it could be cultivated in the liquid within pH3 8,it grew bigger in the pH 7,the total weight of the mycelium up to 1.45 mg·mL -1 ,increase 131.8 times during cultivated period.Among 8 kinds of N sources (ammonium sulfate,ammonium nitrate,calcium nitrate,kalium nitrate,organic urea,ammonium tartrate,glycin,and glutamic acid),ammonium tartrate is the best in absorbing and assimilating this fungus,the total weight is up to 1.684 mg·mL -1 ,153 times of the originality.Next is the glutamic acid.Giving 8 kinds of C (glucose,fructose,sugar,maltose,ethanol,mannaol,dextrin,dissoluble starch)every one could be used ,especially the fructose,the total weight of the mycelium up to 1.501 mg·mL -1 increase by 136 times.Among the sources of phosphor,kalium phosphate with two hydrogen atom (1 mg·mL -1 )is the best,the total weight of the mycelium up to 1.256 mg·mL -1 ,increase by 114.2 times.

关 键 词：石斛 菌根真菌 生长特性 液体培养 

分 类 号：Q949.32[生物学—植物学] S718.81[农业科学—林学]