组织工程化大鼠皮肤的构建  

Reconstruction of tissue engineered rat skin

作　　者：韩运双[1] 浦跃朴[1] 李云晖[1] 尹立红[1] 葛存旺[2] 

机构地区：[1]东南大学公共卫生学院,南京210009 [2]东南大学生物科学与医学工程系,南京210096

出　　处：《东南大学学报（自然科学版）》2002年第3期367-370,共4页Journal of Southeast University：Natural Science Edition

基　　金：国家自然科学基金资助项目 ( 396 70 6 42 );江苏省 333工程基金资助项目

摘　　要：本研究应用组织工程学技术构建了具有完整表皮和真皮结构的组织工程化大鼠皮肤 .在电化学工作站中 ,按照吡咯单体浓度 0 .0 5mol/L、聚合时间 5 0 0s、电流 1 0mA的条件制备聚吡咯膜 .采用胰蛋白酶消化法从新生SD大鼠皮肤中分离获得角朊细胞并种植于聚吡咯膜上 ,导电培养获得较多数量的角朊细胞 .将Ⅰ型胶原蛋白醋酸溶液与大鼠皮肤成纤维细胞相混合培养 1 4d ,形成胶原蛋白成纤维细胞凝胶真皮模型 .将角朊细胞种植于真皮模型上浸没式培养 5d后 ,继续气液面培养 1 4d .经固定、切片与染色 ,在光镜和扫描电镜下可见所构建的真皮模型与新生大鼠真皮具有类似的结构 ;而大鼠皮肤模型则具有与正常新生大鼠皮肤相类似的结构 ,包括完整的真皮层和表皮层 ,表皮中含有完整的基底层、棘细胞层、颗粒层与角质层 .该模型的建立 。A model of tissue engineered rat skin with dermal and epidermal structures is reconstructed. The conductive polypyrrole membranes were made in electrochemical station. The rat keratinocytes were isolated and planted on the membranes and large quantities of undifferentiated keratinocytes were then obtained after 72*!h-culture of electrical stimulation. In the reconstruction of tissue engineered rat dermis, the rat dermal fibroblasts mixed with typeⅠcalf collagen acetic acid solutions were cultured 14 d and a dermal tissue-like structure of fibroblast-populated collagen gel matrix was formed. The reconstructed rat dermis showed a similar morphology to that of normal newborn rat dermis under microscopy. The keratinocytes were then seeded on reconstructed rat dermis. After a period of 5 d submerged culture, the cells were raised to air-liquid interface to culture for 14*!d, a piece of reconstructed rat skin was formed. The reconstructed rat skin was similar in morphology to that of normal newborn rat skin with dermis and epidermis, which contained basal layer, granular layer and stratum corneum. The results are helpful to reconstruction and clinical application of tissue engineered human skin.

关 键 词：构建 组织工程 皮肤 大鼠 角朊细胞 真皮层 表皮层 皮肤移植 体外培养 

分 类 号：Q813.1[生物学—生物工程] R622.1[医药卫生—整形外科]