高效液相色谱法测定奥美拉唑血药浓度及其药代动力学  

Determination of Omeprazole in Plasma and Its Pharmacokinetics by HPLC

作　　者：张静[1] 邵志高[1] 王宇环[2] 杨辉 

机构地区：[1]南京医科大学第一附属医院,南京210029 [2]江苏省中医药研究所,南京210028 [3]连云港海州医院,连云港222000

出　　处：《江苏药学与临床研究》2001年第4期8-10,共3页Jiangsu Pharmacertical and Clinical Research

摘　　要：建立了用反相高效液相色谱法测定血浆中奥美拉唑含量的分析方法。该分法采用液相提取预处理血样,在25～2000 ng/ml的浓度范围,线性关系良好,最低检测浓度4 ng/ml,奥美拉唑的相对回收率(98.07±5.21)％,日内变异<3.0％,日间变异<8.5％。并且,应用本法对正常人静脉推注奥美拉唑进行了药代动力学研究。A reversed-phase HPLC method for quantitative determination of orneprazole in plasma was established. The blood plasma sample detection was pretreated by liquid-liquid extraction. Calibration curves in the range 25-2000 ng/ml for omeprazole were linear. The lower limited concentration was 4 ng/ml. The recovery rate of omeprazole was (98. 07 + 5. 21)%. The intraday and interday cofficients of variation were <3% and <8. 5% respectively. The pharmacokinetic characteristics of omeprazole in normal subjects after a single iv administered were investigated by the method.

关 键 词：奥美拉唑 高效液相色谱法 药代动力学 含量测定 

分 类 号：R975.6[医药卫生—药品] R927.2[医药卫生—药学]