荧光定量聚合酶链反应检测淋病奈瑟菌  

Fluorogenic Quantitative PPCR Method for Detecting Neisseria Gonorrhoeae

作　　者：袁应华[1] 陈文雨[2] 黄强[1] 张文芳[1] 

机构地区：[1]同济大学附属同济医院检验科,上海200065 [2]中山医科大学达安基因诊断中心,广州510089

出　　处：《同济大学学报（医学版）》2002年第2期156-157,共2页Journal of Tongji University(Medical Science)

摘　　要：目的　评价荧光定量聚合酶链反应 (FQ -PCR)技术检测淋病奈瑟菌的临床应用。方法　采用FQ -PCR技术检测疑似淋病患者泌尿生殖道标本 80例 ,并同时进行淋病奈瑟菌培养 ,对两种方法的检测结果进行比较分析。结果　测试 80例标本 ,FQ -PCR法 30例为阳性 ,DNA拷贝数从 3× 10 5/ml至 6× 10 10 /ml不等 ,阳性率为 37.5 %(30 / 80 ) ;培养法 2 4例阳性 ,阳性率 30 .0 % (2 4/ 80 ) ;FQ -PCR较培养法差异有显著性 (P <0 .0 0 5 )。结论　FQ -PCR技术可定量检测标本中的DNA拷贝数 ,较培养法简单、敏感、准确 ,对于淋病的早期确诊 。Objective To evaluate the fluoreeogenic quantitative PCR(FQ?PCR) method for detecting Neisseria gonorrhoeae in clinical samples.Methods 80 urogenital secretion samples were identified by FQ?PCR and culture method respectively. The results were analyzed and compared.Results In 80 samples, the DNA copies of Neisseria gonorrhoeae was 3.0×10 5/ml to 6.0×10 10 /ml, with a positive rate of 37.5% (30/80) by using FQ?PCR, and 30.0% (24/80) by culture method ( P <0.005).Conclusion Compared with culture method, FQ?PCR method for detecting Neisseria gonorrhoeae is more simple, sensitive, and accurate. Its clinical significance in early diagnosis and in evaluating the effectiveness of the treatment of urogenital infections is obvious.

关 键 词：淋病奈瑟菌 聚合酶链反应 荧光光度测定法 

分 类 号：R378.16[医药卫生—病原生物学]