猪资源家系MC4R基因扫描及其与脂肪性状的相关分析  

Molecular Screening of MC4R Gene and Association with Fat Traits in Pig Resource Family

作　　者：刘桂兰[1] 蒋思文[1] 熊远著[1] 郑嵘[1] 屈彦纯[1] 

机构地区：[1]华中农业大学农业部猪遗传育种重点开放实验室,武汉430070

出　　处：《Acta Genetica Sinica》2002年第6期497-501,共5页

基　　金：国家"973"(G2 0 0 0 0 1610 5 );"863"(2 0 0 1AA2 13 12 1)资助项目~~

摘　　要：黑素皮质激素受体 4 (Melanocortin 4Receptor,MC4R)是在人类肥胖研究中发现的重要调节因子 ,它可以与瘦蛋白 (Leptin)、神经肽Y(NeuropeptideY ,NPY)、α 黑素细胞刺激激素 (Alpha melanocyte stimulatinghormone ,α MSH)等一起调节动物体重和采食量。采用PCR RFLP技术 ,分析了MC4R基因部分片段在猪资源家系群体中的TaqⅠ酶切片段多态性分布。MC4R基因多态性与生长肥育性状、肉质性状、胴体性状的相关性分析的结果表明 ,MC4R基因型频率在不同品种群体中的分布不同 ;MC4R基因与猪胸腰椎间膘厚、臀部膘厚、平均背膘、眼肌宽度、眼肌面积、皮率呈显著性相关。MC4R基因主要以显性作用方式发挥作用 。Melanocortin 4 Receptor (MC4R) plays an important role in the regulation of human obesity. It can cooperate with leptin, neuropeptide Y(NPY) and melanocyte stimulating hormone (MSH) to regulate body weight and feeding. Inactivation of this receptor by gene targeting in mice results in a maturity onset obesity syndrome associated with hyperphagic,hyperinsulinemia, hyperglycemia, as well as decreased linear growth and adult obesity.Mutiple alignments of the sequences from individuals of several pig lines identified a single nucleotide substitution(G→A) at position 298 of the seventh transmembrane domain. In present study, polymorphism distribution of MC4R gene fragment in resource population was studied using PCR RFLP method based on the enzyme Taq Ⅰ. The genotype was analyzed with the phenotype of the slaughtered individuals. The results showed that the frequencies of MC4R genotype varied in different breeds. The correlation analysis demonstrated the genotype of MC4R was in significant relation with back fat thickness on thorax waist, buttock and the average back fat thickness, as well as with the width and area of longissmus dorsi (LD), and the percentage of skin. MC4R gene plays a role mainly in the pattern of dominant effect, and all the additive effects were not significant.

关 键 词：猪资源家系MC4R 基因扫描 脂肪性状 相关分析 背膘厚 

分 类 号：S852[农业科学—基础兽医学]