白菜类蔬菜遗传多样性的AFLP分子标记研究  

Genetic Diversity in Brassica rapa Revealed by AFLP Molecular Markers

作　　者：郭晶心[1] 周乃元[1] 马荣才[1] 曹鸣庆[1] 

机构地区：[1]北京农业生物技术研究中心,北京100089

出　　处：《农业生物技术学报》2002年第2期138-143,共6页Journal of Agricultural Biotechnology

基　　金：北京市科委资助项目(954410900)。

摘　　要：首次对白菜(Brassicarapa)类蔬菜的遗传多样性和分类进行了AFLP分子标记研究。共采用了137份材料,从64对引物组合中选择了4对扩增条带多、多态检出率高的3+3引物组合,检测了210个位点,并对AFLP数据进行了聚类分析。结果表明,芜菁和白菜类蔬菜各自单独聚为一类,它们的亲缘关系较远。在白菜类蔬菜当中,所有大白菜和薹菜聚为一类,它们是比较特殊的类群,且亲缘关系密切。小白菜的聚类结果与其园艺学分类差别较大。小白菜各类型间的相似性程度较低,说明小白菜的起源较大白菜要早。大白菜的聚类表明,大白菜现有品种的遗传多样性较为狭窄。AFLPtechnique,followedby thedetectionusingthefluorescentsystems,hasbeenusedto explorethegeneticdiversity andtaxonomyof137accessionsof Brassica rapa collectedfromdifferentpartsof Chinaandforeigncountries.Fourpairsof3+3primercombinationproducinghigh polymorphismand powerfuldistinctivenesshave been selectedfrom64pairsof primer combination.A totalof210lociweredetectedandclusteranalysiswas carriedout.Allaccessionsweregroupedintotwo separate groups,oneincluding allturnipaccessionsandanotherallChinesecabbageaccessions.TheheadingChinesecabbageandtheTaicai,a typeof non-headingChinesecabbage,formedoneuniquesubgroupin thegroupof Chinesecabbage.Thissuggestedthatturnipand Chinesecabbagebelongto two differentsubspeciesof Brassica rapa andtheheadingChinesecabbageis a specialvarietywithinthe subspecies.TheTaicaihasa closerelationshipwiththeheadingChinesecabbageandmaybe theitsancestor. Clusteringresultsof non-headingChinesecabbagewerecomplexandshowedmuchdeviationfromthoseof horticulturaltaxonomy. Thesimilarityamong non-headingChinesecabbagetypeswaslowerthanthatamongtheheadingChinesecabbage,suggestingthatthenon-headingChinese cabbageoriginatedearlierandhada longerevolutionhistory.The highlevelsimilarityof Chinesecabbagesuggestedthatit was necessaryto findnewgermplasmresourcesfromprimitivetypesof headingChinesecabbageforitsgeneticimprovement.

关 键 词：白菜类蔬菜 遗传多样性 AFLP分子标记 遗传多样性 大白菜 小白菜 

分 类 号：S634.03[农业科学—蔬菜学]