HORMONAL CONTROL OF THE VITELLOGENESIS IN THE JAPANESE OAK SILKWORM, ANTHERAEA YAMAMAI (LEPIDOPTERA : SATURNIIDAE)  

天蚕卵黄发生的激素调控(英文)

作　　者：叶恭银[1] 胡萃[1] 龚和[2] 

机构地区：[1]浙江大学应用昆虫学研究所,杭州310029 [2]中国科学院动物研究所,北京100080

出　　处：《Entomologia Sinica》2002年第1期17-25,共9页中国昆虫科学（英文版）

基　　金：中国国家自然科学基金 (项目号 :3930 0 0 18)

摘　　要：Effects of ecdysteroid and juvenile hormone (JH) on vitellogenesis of the Japanese oak silkworm, Antheraea yamamai are reported in this article. After topical treatment with 20 hydroxyecdysone alone or JH analog (i.e. methoprene) alone and combined treatment with these two chemicals, vitellogenin (Vg) titers in the fat body and haemolymph at the pupal stage were mostly higher than those of the control, indicating that both ecdysteroid and JH exerted a promoting effect on the synthesis of Vg. In contrast, the Vg uptake was markedly inhibited by JH while stimulating effect of the ecdysteroid could be shown that vitellin (Vt) titer in the ovary was lower after methoprene treatments, but higher after 20 hydroxyecdyson treatments. Meanwhile, effects of these two hormones on Vg synthesis in the fat body were also tested with the incubation in vitro with Grace medium containing 3H leucine and the hormones. The results demonstrated that Vg synthesis was stimulated after treating with methoprene alone or 20 hydroxyecdysone alone and combined treating with these two chemicals, and particularly ecdysteroid had more marked positive effect. To comprehensively concluded our results, it could be regarded that ecdysteroid play the main role in the regulation of vitellogenesis for the Japanese oak silkworm.报告了蜕皮激素和保幼激素对天蚕Antheraeayamamai卵黄发生的调控作用。当单独以 2 0 羟基蜕皮酮或保幼激素类似物methoprene处理 ,以及同时用这两种激素处理天蚕蛹时 ,蛹期脂肪体和血淋巴中卵黄原蛋白(Vg)含量明显高于对照 ,即二者对Vg的合成起促进作用。然而 ,卵巢中卵黄蛋白 (Vt)含量则因激素种类而异 ,以保幼激素处理时明显低于对照 ,以 2 0 羟基蜕皮酮处理则反之 ,即前者抑制卵巢对Vg的摄取 ,而后者则起促进作用。离体培养脂肪体并以激素处理的结果表明 ,2 0 羟基蜕皮酮和methoprene均能促进Vg合成 ,但前者作用更明显。综合考虑上述结果可以认为蜕皮激素对该蚕的卵黄发生起主要调控作用。

关 键 词：Antheraea yamamai VITELLOGENESIS juvenile hormone ECDYSTEROID 

分 类 号：S885.3[农业科学—特种经济动物饲养]