微量滴定板法测定蚊虫非特异性酯酶检测抗药性的研究  

Microplate Test of Non-specific Esterase of Mosquito for Insecticide Resistance Detection

作　　者：李士根[1] 蒋滨[2] 甄天民[3] 王凤刚[1] 刘永春[1] 孙传红[3] 王怀位[3] 

机构地区：[1]济宁医学院寄生虫学教研室,济宁272013 [2]济宁市中区人民医院 [3]山东省寄生虫病防治研究所

出　　处：《中国媒介生物学及控制杂志》2002年第3期178-180,共3页Chinese Journal of Vector Biology and Control

基　　金：山东省卫生厅;济宁市科委资助项目 (No .99- 2 - 2 9-3)

摘　　要：目的 :探讨简捷灵敏的蚊虫抗药性早期测报技术。方法 :以 β -乙酸萘酯为底物 ,坚固蓝B盐为显色剂 ,用微量滴定板法测定实验室和现场单个淡色库蚊非特异性酯酶活力。结果 :实验室 5个品系淡色库蚊以抗DDVP品系非特异性酯酶活力水平最高 ,其次为抗残杀威品系和抗DDVP降解品系 ,抗氯氰菊酯品系较低 ,与敏感品系相近 ;3个现场淡色库蚊种群对DDVP抗性为 4.15～ 9.3 6倍 ,对残杀威抗性为 1.0 2～ 3 .81倍 ,其非特异性酯酶活力水平均较高。结论 :微量滴定板法测定非特异性酯酶能够检测蚊虫对有机磷和氨基甲酸酯类杀虫剂的抗性水平。方法简便快速 ,结果客观准确 ,可重复性强 ;可测出抗性频率 ,早期发现抗性的存在 ;可测知抗性机制 。Objective:To explore simple and sensitive technic for insecticide resistance detection and early prediction.Methods:Microplate test was used to detect non-specific esterase (NSE) of single Cx.pipeins pallens of laboratory and field population, using β-Naphthyl acetate as the substrate and fast blue B salt solution as the colour agent. Results:The NSE level was highest in anti-DDVP strain, followed by anti-propoxur strain and anti-DDVP degenerated strain, while the level was similar between anti-cypermethrin and susceptible strain. Three field collected Cx.pipiens pallens populations have 4.15 - 9.36 fold higher resistance against DDVP and 1.02 - 3.81 fold higher resistance against propoxur than susceptible mosquito, and their NSE level was also higher than susceptible strain. Conclusion:Microplate test for NSE can be used for the detection of resistance against Op and carbamate insecticides. The method is easy and rapid to perform with high precision and objective results. Resistance frequency and mechanism can be detected at the same time, so early prediction of resistance and working out resistance management measure is possible.

关 键 词：微量滴定板法 测定 蚊虫 非特异性酯酶 检测 抗药性 

分 类 号：R384.1[医药卫生—医学寄生虫学] R184.31[医药卫生—基础医学]