中华眼镜蛇毒神经生长因子对神经元的保护作用  

Protective effect of nerve growth factor (NGF) from the Chinese cobra venom on spinal neurons after sciatic nerve lesion

作　　者：陈敏坚[1] 杨斌[1] 张春燕[1] 舒雨雁[1] 

机构地区：[1]广西医科大学一附院临床医学实验中心,南宁530021

出　　处：《中国临床药理学与治疗学》2001年第1期5-7,W003,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：广西壮族自治区科学基金资助(桂科自9811039;9518002)

摘　　要：目的探讨中华眼镜蛇毒神经生长因子对受损神经元的保护作用。方法钳夹损伤成年大鼠坐骨神经 ,造成脊髓背根神经节感觉神经元和脊髓前角运动神经元变性的动物模型 ,用酶组织化学图像分析观察神经生长因子对受损神经脊髓节段的抗氟化物酸性磷酸酶和乙酰胆碱酯酶活性的影响。结果神经生长因子能明显提高受损神经脊髓节段的抗氟化物酸性磷酸酶和乙酰胆碱酯酶活性。Aim To investigate the protective effect of nerve growth factor (NGF) from the Chinese cobra venom on spinal neurons after sciatic nerve lesion.Methods Fourty Wistar rats were divided into 2 groups each containing 20 animals. After surgical crush lesion on the bilateral sciatic nerve,the rats were injected NGF into the target muscle every day.On the d 5 and d 10 after operation,enzyme histochemistry technique was used to show acetycholinesterase (AchE) activity of the motoneurons in L5～S2 spinal cord anterior horn and fluoride resistant acid phophatase(FRAP) activity of substantia gelatinosa in L5～S2 spinal cord posterior horn. The activity of enzyme was analysed with computer image analysis system.Results Compared with the control group, the activity of FRAP in NGF group was higher on the d 5 and d 10 after operation and the activity of AchE in NGF group was higher on the d 10 after operation.Conclusion It is demonstrated that NGF could protect the mononeurons in spinal cord anterior horn and the sensory neurons in dorsal root ganglia after axotomy.

关 键 词：神经生长因子 坐骨神经 图像分析 

分 类 号：R965[医药卫生—药理学]