四甲吡嗪对人类白细胞呼吸爆发氧代谢的影响(英文)  

Influence of tetramethylpyrazine on oxygen metabolism during the respiratory burst of human neutrophils

作　　者：张兆辉[1] 余绍祖[1] 赵保路[2] 

机构地区：[1]湖北医科大学附属第一医院,湖北神经精神病研究所武汉430060 [2]中国科学院生物物理研究所,北京100080

出　　处：《中国临床药理学与治疗学》2001年第1期8-11,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：Keyprojectsupportedby HubeiCommitteeforScienceandTechnology,№981P1602

摘　　要：目的研究四甲吡嗪 (川芎嗪 ,Lig)对白细胞呼吸爆发期间氧消耗和过氧化物的影响。方法采用电子自旋共振自旋捕集技术 ,自旋探针氧测定法和化学发光法检测白细胞呼吸爆发期间氧消耗和活性氧自由基。结果Lig对白细胞呼吸爆发期间的氧消耗无明显影响 (P>0.05),但能明显抑制其产生的化学发光 (P<0.01) ,这是Lig清除O2· 和OH·的结果。结论LigAim To investigate the influence of tetramethylpyrazine (ligustrazine, Lig) on oxygen consumption and superoxide during the respiratory burst of human neutrophils.Methods It was observed by using ESR spin trapping,spin probe oxymetry and luminol_dependent chemiluminesence (CL).Results Lig had no influence on oxygen consumption during the respiratory burst of neutrophils (P>0.05), but had remarkable inhibition on CL response generated by neutrophils(P<0.01), and had scavenging effect on and OH· generated by neutrophils, which were demonstrated in xanthine/xanthine oxidase system and Fentons reaction(P<0.01). Conclusion Lig has no inhibiting effect on oxygen metabolic function of neutrophils,but protects tissue from injury caused by activated neutrophils through scavenging oxygen radicals.

关 键 词：吡嗪 川芎嗪 电子自旋共振 化学发光 氧自由基 抗氧化剂 

分 类 号：R282.7[医药卫生—中药学]