黄芪总甙的抗炎作用及其作用机制探索  

Anti-inflammatory effect of astragalosides and its mechanisms of action

作　　者：杨沁[1] 路景涛[1] 王斌[1] 周爱武[1] 陈敏珠[1] 

机构地区：[1]安徽医科大学临床药理研究所

出　　处：《中国临床药理学与治疗学》2001年第1期21-24,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的探索黄芪总甙 (AST)的抗炎作用及其作用机制。方法采用大鼠角叉菜胶气囊炎症模型 ,测定渗出液量、中性白细胞游出数、蛋白质、PGE2、IL_8、NO、PLA2 含量以及O2· 的生成量。结果AST40、80mg·kg-1 可使角叉菜胶诱导大鼠气囊炎症的渗出液量、中性白细胞游出数、蛋白质含量显著减少,降低渗出液及中性白细胞中PLA2 活性 ,减少渗出液中IL_8含量及中性白细胞O2· 的生成。AST也可明显减少渗出液中PGE2、NO的含量。结论AST的抗炎作用机理与其降低血管通透性和抑制白细胞游出、降低PLA2 活性、减少IL_8、PGE2。Aim To study the anti_inflammatory effect of astragalosides(AST) and its mechanisms of action.Methods The exudate volume, neutrophil count and protein content in exudate were measured in the carrageenan_induced air pounch model in rats. The content of PGE2 was assayed by radioimmunoassay,the activity of PLA2 by microacid titration assay, IL_8 by ELISA, and NO by nitrate reductase assay.The production of in neutrophil was determined by cytochrome C assay. Results AST (40,80 mg·kg-1) could markedly reduce the exudate volume, neutrophil count, protein content, the content of IL_8, and the production of .AST lowered PLA2 activity of neutrophil and accellular component in exudate, and it also decreased the contents of PGE2 and NO in exudate. Conclusion AST has an obvious anti_inflammatory effect on carrageenan_ induced acute inflammation. Its mechanisms may be related to the inhibition of vascular permeability and leukocyte migration, as well as to the suppression of PLA2 activity and the reduction of IL_8,PGE2,NO and production.

关 键 词：黄芪总甙 角叉菜胶 抗炎作用 白细胞介素-8 磷脂酶A2 

分 类 号：R967[医药卫生—药理学]