猕猴桃属植物线粒体DNA片段PCR-RFLP研究初报  

PCR-RFLP Analysis on Mitochondrial DNA of Actinidia

作　　者：李林林[1] 黄宏文[1] 

机构地区：[1]中国科学院武汉植物研究所,武汉430074

出　　处：《武汉植物学研究》2002年第2期153-156,共4页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金资助项目 (3 0 0 70 0 82 );中国科学院资源与生态环境研究重点项目 (KZ95 2 -J1-10 2 );中国科学院生命科学与生物技术青年科学家小组资助;欧盟项目 (INCO-DC IC18CT970 183 );国家重点基础研究发展规划项目 (G2 0 0 0 0 4680 6)

摘　　要：Thirty four taxa of Actinidia were investigated through PCR RFLP analysis of two mitochondrial DNA fragments, including nad1/B C gene and nad4/1 2 gene. Digestion of these two fragments by seven restriction endonucleases yielded twenty polymorphic restriction fragments. We only found restriction fragment length variation caused by insertion or deletion. It suggests that mtDNA restriction length polymorphism could be an efficient indicator for conservation biology .Thirty four taxa of Actinidia were investigated through PCR RFLP analysis of two mitochondrial DNA fragments, including nad1/B C gene and nad4/1 2 gene. Digestion of these two fragments by seven restriction endonucleases yielded twenty polymorphic restriction fragments. We only found restriction fragment length variation caused by insertion or deletion. It suggests that mtDNA restriction length polymorphism could be an efficient indicator for conservation biology .

关 键 词：猕猴桃属 线粒体DNA PCR-RFLP 单元型 

分 类 号：Q949.758.2[生物学—植物学] Q943.2