TGF-β对胎兔颅骨成骨样细胞体外成骨作用及BMP蛋白表达影响的研究  

Effects of TGF-β on osteogenosis and BMP expression of primary osteoblast-like cells derived from fetal rabbit calvarium in vitro

作　　者：唐康来[1] 杨柳[1] 许建中[1] 卢卫忠[1] 吴梅英[1] 李起鸿[1] 

机构地区：[1]第三军医大学附属西南医院骨科,重庆400038

出　　处：《第三军医大学学报》2002年第5期547-549,共3页Journal of Third Military Medical University

摘　　要：目的　探讨TGF β对胎兔颅骨成骨样细胞体外成骨作用及BMP蛋白表达的影响。 方法　采用ALP活性测定及骨结节计数方法观察TGF β对胎兔颅骨成骨样细胞体外的成骨作用 ,BMP McAb免疫组化染色及其定量分析观察TGF β对胎兔颅骨成骨样细胞BMP蛋白表达的影响。结果　TGF β( 3～ 7d)对ALP活性有促进作用 ,并呈一定的时效和量效关系。TGF β对骨结节形成低剂量时有促进作用 ,高剂量时有较强的抑制作用 ;TGF β对成骨细胞BMP蛋白表达表现为抑制作用 ;二者均有显著性差异。 结论　TGF β对胎兔颅骨成骨样细胞体外成骨及BMP蛋白表达表现为双相作用。Objective To explore the effects of TGF-βon the osteogenosis and the expression of bone morphogenetic protein (BMP) of primary osteoblast-like cells derived from fetal rabbit calvarium in vitro . Methods The effects of TGF-β on the osteogenosis of primary osteoblast-like cells were investigated with alkaline phosphatase activity assay and bone nodule counting. The effects of TGF-β on BMP expression were tested with immunohistochemical staining and quantitative analysis. Results TGF-β given for 3-7 d increased ALP activity in a dose- and time-dependent manner. It was found TGF-β enhanced the bone nodule formation at low concentration, but inhibited it at high concentration. TGF-βinhibited the BMP protein expression, which increased along with the dose. Conclusion TGF-β showed bi-directional effects on the osteogenesis and BMP protein expression of rabbit osteoblast-like cells derived from fetal calvarium in vitro .

关 键 词：胎兔 成骨样细胞 转化生长因子Β M有形态发生蛋白 骨形成 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R392.11[医药卫生—基础医学]