DDPH对HECCM促PASMC增殖后的逆转效应研究  

Study on the Reversible Effects of DDPH on Post-Proliferation of Pulmonary Arterial Smooth Muscle Cells Induced by Hypoxia Endothelia Cell Conditioned Medium

作　　者：张红胜[1] 王西明[1] 陈蓓蓓[1] 雷景迈[1] 何善述[1] 

机构地区：[1]华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2002年第3期234-237,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No. 392 70 2 95 )

摘　　要：利用低氧内皮细胞条件培养基 (HECCM)建立猪肺动脉平滑肌细胞 (PASMC)的增殖模型 ;以四甲基偶氮唑盐 (MTT)比色法、α平滑肌肌动蛋白 (α- SM- actin)为指标 ,用免疫细胞化学染色及透射电镜等方法观察不同浓度DDPH[1- (2 ,6 - dim ethylphenoxy) - 2 - (3,4 - dimethoxy phenylethylamino) propane hydrochloride]对 HECCM促 PASMC增殖后的逆转效应。结果表明 :HECCM促 PASMC增殖后 ,PASMC的表型发生转化 ,由收缩表型转化为合成表型 ,PASMC胞质内的 α- SM- actin含量下降 ;而 DDPH能使 HECCM促 PASMC增殖后的表型逆转 ,即由合成表型逆转为具有执行正常收缩功能的收缩表型 ,PASMC胞质内的 α- SM- actin含量回升 ;且 DDPH的这种作用具有浓度相关性。透射电镜结果从形态学上也证明 PASMC由合成表型逆转为收缩表型 ,由此推测 ,DDPH对 HECCM促A proliferative model of pulmonary arterial smooth muscle cells (PASMC) was developed by using hypoxial endothelial cell conditioned medium. By using cell culture, MTT colorimetry, immunocytochemistry and transmission electron microscopy (TEM), the effects of hypoxia endothelia cell conditioned medium on the PASMC proliferation and the reversible effects of DDPH at different concentrations on the post proliferation of PASMC were observed. The results showed that PASMC phenotypes changed from the contractile phenotype to the synthetic phenotype after HECCM promoting PASMC proliferation, and α SM actin level was decreased accordingly. DDPH, in a dose dependent manner, not only could inhibit PASMC proliferation, but also could transform the synthefic phenotype of PASMC at post proliferation to contractile phenotype, and the α SM actin level was increased simultaneously. Transmission electron microscopic examination also verified the changes of the phenotypes. It was postulated that DDPH could reverse the effect of HECCM promoting PASMC proliferation.

关 键 词：DDPH HECCM PASMC增殖 逆转效应 丙烷盐酸盐 Α-平滑肌肌动蛋白 降压药 

分 类 号：R322.1[医药卫生—人体解剖和组织胚胎学] R972.4[医药卫生—基础医学]