高密度脂蛋白和载脂蛋白AⅠ促进单核细胞源性巨噬细胞产生前列腺素E_2  

High Density Lipoprotein and Apolipoprotein AⅠ Enhance PGE_2 Production by Monocyte-derived Macrophage

作　　者：周小琴[1,2] Arnold von Eckardstein 

机构地区：[1]华中科技大学同济医学院附属协和医院内科 [2]德国明斯特大学临床化学与实验医学研究所,德国明斯特D-48149

出　　处：《华中科技大学学报（医学版）》2002年第3期284-286,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：前列腺素 E2 (PGE2 )是巨噬细胞产生的二十烷酸代谢的主要产物之一。为探讨高密度脂蛋白 (HDL )和载脂蛋白 A (apo A )抗动脉硬化的作用机制 ,观察了二者对单核细胞源性巨噬细胞产生 PGE2 的影响。培养的人外周血单核细胞源性巨噬细胞先经过脂质负载 ,再分别与 HDL3和 apo A 孵育 ;利用酶联免疫测定法检测细胞培养物上清液中的 PEG2 含量。结果显示 :孵育 2 4 h后 HDL3组细胞合成与分泌的 PGE2 是对照组的 370 % ,apo A 也增加巨噬细胞 PGE2的产生。HDL3的作用明显大于 apo A ,且呈时间依赖性。表明 HDL3和无脂质 apo A 都可以促进巨噬细胞产生 PGE2 ,这可能参与 HDL和 apo AThe effect of antiatheronic high density lipoprotein (HDL) and apolipoprotein (apo) AⅠ on production of prostaglandin E 2 (PGE 2) by human monocyte derived macrophage was investigated. Macrophages were loaded with acetylated low density lipoprotein for foam cells formation followed by incubation with RPMI 1640 alone (control), HDL 3 or apoAⅠ. PGE 2 production in cell culture supernatant was measured by enzyme immunoassay. At 24 h of incubation, PGE 2 synthesized and secreted by the cells incubated with HDL 3 was increased to 370 % of the control. ApoAⅠ also increased PGE 2 production by the macrophages. The effect of HDL 3 was stronger than apoAⅠ in a time dependent manner. It was demonstrated that both HDL 3 and lipid free apoAⅠ enhance PGE 2 synthesis and secretion by human monocyte derived macrophages, which might be involved in the protection of HDL and apoAⅠ from atherosclerosis.

关 键 词：动脉粥样硬化 高密度脂蛋白 载脂蛋白AⅠ 单核细胞源性 巨噬细胞 前列腺素E2 

分 类 号：Q513.5[生物学—生物化学] R543.5[医药卫生—心血管疾病]