银杏叶提取物对豚鼠腹腔神经节细胞生物电的影响  

The electrophysiological effects of ginkgo biloba extract on the neurons of the guinea-pig celiac ganglia in vitro

作　　者：张玲[1] 胡金兰[1] 孔德虎[1] 黄振信[1] 王烈成[1] 刘芳[1] 祝延[1] 

机构地区：[1]安徽医科大学生理学教研室,合肥230032

出　　处：《安徽医科大学学报》2002年第2期99-102,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅科研基金资助课题 (编号 :2 0 0 2KJ14 7)

摘　　要：目的　通过观察银杏叶提取物 (EGb)对交感神经节细胞生物电的影响 ,为进一步探讨EGb调节包括血管运动在内的内脏功能活动的机制提供依据。方法　细胞内生物电记录技术和灌流给药法。结果　EGb(5 0～ 5 0 0mg/L)可使 75 0 %的腹腔神经节 (CG)细胞产生去极化反应 ,幅度(5 5 8± 0 79)mV ,时程 (2 40 7± 5 0 3)s,且表现剂量依赖性 ;12 5 %的细胞超级化 ;6 3%出现先超级化再去极化的双相反应 ;另有 6 3%的细胞在相同的灌注条件下 ,于不同的时间内 ,分别出现超级化和去极化两种反应。去极化反应的细胞其膜电阻表现为降低 (33 8%)、增大 (2 6 2 %)和不变(4 0 0 %)。此去极化反应可被低钙 /高镁克氏液部分阻断 ,但不被胆碱和肾上腺素受体阻断剂所阻断。EGb尚可抑制外源性Ach引起的去极化电位。结论　EGb可能通过影响肽类递质的释放 ,进而影响豚鼠CG细胞生物电活动。Objective To investigate the electrophysiological effect of extract of ginkgo biloba(EGb) on the neu-rons of the guinea-pig celiac ganglia in vitro. Methods The intracellular recording technique and the administration of superfusion.Results Superfusion of ceoliac ganglia with EGb(50～500 mg/L) evoked a concentration-dependent membrane depolarization in the majority(75.0%) of the neurons, with the mean amplitude and the average duration of (5.58±0.79) mV and (240.7±50.3) s respectively. 12.5% of the cells were induced hyperpolarization and 6.3% biphasic response which consisted of an initial hyperpolarization phase and a followed depolarization. 6.3% of the cells showed both depolarization and hyperpolarization at different time and under the same condition. The Rm which accompanied by depolarization decreased in 33.8%, increased in 26.2% and unchanged in the rest 40.0%. Depolarizations of EGb could be partially eliminated by a low Ca 2+ and high Mg 2+ solution but not affected by cholinergic-receptor and adrenoa-receptor antagonists. EGb also attenuated the depolarization caused by applied Ach. Conclusion EGb has an electrophysiological effect on the neurons of celiac ganglia probably by modulating the release of peptide.

关 键 词：银杏叶提取物 豚鼠 腹腔神经节细胞 生物电 影响 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]