柴胡皂甙d(SSd)对K562细胞凋亡及基因表达影响的研究  

EFFECTS OF SAIKOSAPONIN-D (SSd) ON APOPTOSIS AND GENE EXPRESSION OF K652 CELLS

作　　者：夏薇[1] 崔新羽[1] 陈静波[1] 

机构地区：[1]北华大学医学院检验系,吉林132001

出　　处：《中国现代医学杂志》2002年第10期21-23,共3页China Journal of Modern Medicine

基　　金：吉林市科委科研基金资助项目 ( 2 0 0 0 8)

摘　　要：目的 :观察从柴胡中提取的单体成分柴胡皂甙d诱导白血病细胞株K562细胞凋亡及对其基因表达的影响。方法 :分别用 5μg/ml、7.5μg/ml、1 0 μg/ml浓度的SSd作用于K562细胞株 ,于药物作用后不同时间段 (1 2 ,2 4 ,36 ,48,60h) ,荧光染色法观察基凋亡状况、间接免疫荧光法测Bcl- 2表达情况。结果 :荧光染色结果显示 :K562在 1 0 μg/ml处理 2 4h后 ,开始出现凋亡小体 ,48h后出现大量死细胞。间接免疫荧光法测Bcl- 2表达 ;经SSd作用后K562细胞Bcl-Objective:To research the effect of SSd on the cell apoptosis and gene expression of K562 cells. Method:Apoptosis of K562 cells was analyzed by fluorescent stain technique and the expression of Bcl-2 was determined by immunoflourescent antibody technique after the treatment of different density of SSd (5μg/ml?7.5μg/ml?10μg/ml) at different time (12,24,36,48,60h).Results:The fluorescent stain showed that after 24 hours' treatment with 10μg/ml SSd,apoptosis bodies appeared in the K562 cells.After 48 hours,a great deal of dead cells appeared. The expression of Bcl-2 was decreased by ssd.Conclusion:SSd could down-regulate the expression of Bcl-2 and induce apoptosis of K562 cell lines.

关 键 词：柴胡皂甙D K562细胞 影响 细胞凋亡 基因表达 中药 白血病 

分 类 号：R273.37[医药卫生—中西医结合] R282.71[医药卫生—中医肿瘤科]