染铝大鼠学习记忆及脑单胺类神经递质含量的变化  

Changes of learn memory and contents of brain monoamine neurotransmitter in rats with aluminum exposure

作　　者：唐焕文[1] 韦小敏[1] 黄彦妮[1] 谢佩意[1] 王清海 

机构地区：[1]广西医科大学公共卫生学院卫生毒理学教研室,广西南宁530021 [2]广西平果铝业公司职工医院,531400

出　　处：《中国职业医学》2002年第3期17-19,共3页China Occupational Medicine

基　　金：广西壮族自治区科学技术基金 (2 0 0 1 - 1 8)

摘　　要：目的　观察三氯化铝 (AlCl3 )对大鼠学习记忆及脑中枢单胺类神经递质含量变化的影响 ,为探讨铝的神经毒性作用机制提供依据。方法　 14 4只Wistar大鼠随机分为注射剂量分别为 5 0、15 0、2 5 0mg/kg的 3个剂量组和生理盐水对照组。在染毒 4、8、12周时分批进行大鼠跳台实验 ,用荧光法测定各脑区内单胺类神经递质含量。结果　各时间剂量组大鼠主动回避次数显著下降 ,被动逃避延迟时间显著延长 ,差异均有显著性 (P <0 0 1) ;各时间剂量组多巴胺 (DA)和去甲肾上腺素 (NE)含量均呈梯度下降趋势 ,差异有显著性 (P <0 0 1)。除 5 0mg/kg组 4周和 8周时大脑 5 羟色胺 ( 5 HT)和海马 5 羟吲哚乙酸 ( 5 HIAA)含量无变化外 ,其他各时间剂量组的 5 HT和 5 HIAA含量均呈梯度下降 ,差异均有显著性 (P <0 0 1)。结论　实验结果表明 ,铝可影响学习记忆能力 ,铝能导致各脑区单胺类神经递质含量下降且作用部位有选择性 。Objective To observe the effect of aluminum on the learn memory and contents of monoamine neurotransmitter in rats brain and to provide evidence on the mechanism of neurotoxicity. Methods 144 Wistar rats were divided into three experimental and one control group, experimental groups were intraperitoneally injected with aluminum chloride in 5.0, 15.0 and 25.0 mg/kg respectively and control group intraperitoneally injected with N.S for several weeks. Step-down test was performed and then killed, the contents of monoamine neurotransmitter in rat brain was measured with Fluorescene Spectrophotometer. Results Aluminum could decrease active avoidance response and retard passive avoidance time significantly (P< 0.01); DA and NE levels were less than those of control group significantly (P< 0.01); 5-HT and 5-HIAA levels in all dose groups were less than those in control group significantly (P< 0.01) except 5-HT levels in cerebrum and 5-HIAA levels in hippocampus levels at dose of 5.0 mg/kg during the 4th and the 8th weeks.Conclusion It suggested that aluminum could have influence on learn memory and could decrease monoamine neurotransmitter levels in different brain regions. It might be one of the mechanisms of aluminum neurotoxicity.

关 键 词：染铝 大鼠 学习记忆 脑 单胺类神经递质 含量 

分 类 号：R135.1[医药卫生—劳动卫生] R994.3[医药卫生—公共卫生与预防医学]