铅致新生大鼠脑氧化损伤及细胞凋亡的某些改变  

Changes of certain indexes of oxidative damage and apoptosis in brain of rat offsprings with lead exposure

作　　者：逯晓波[1] 陈葆春 于丽华 李北利[1] 刘秋芳[1] 蔡原[1] 

机构地区：[1]中国医科大学公共卫生学院 [2]辽宁省卫生监督所,110005 [3]辽宁省肿瘤研究所,110042

出　　处：《中国职业医学》2002年第3期34-36,共3页China Occupational Medicine

摘　　要：目的　探讨发育期铅暴露所致神经毒性机制。方法　母鼠从怀孕始至仔鼠出生后 7d染铅致新生大鼠染铅模型 ,测定新生大鼠血、脑铅含量 ;同时测定脑组织脂质过氧化物 (LPO)水平、谷胱甘肽 (GSH)含量及超氧化物歧化酶(SOD)活性 ;另外应用原位末端标记TUNEL法测定脑组织海马区及其附近皮质细胞凋亡数量。结果　染铅使出生后 7d幼鼠脑LPO水平升高 ,GSH含量下降 ,SOD活性降低 ,且海马区附近皮质细胞凋亡数量增加 (高剂量染铅组每计数 10 0个细胞中凋亡细胞数为 2 2 6 7± 4 0 3,而对照组为 17 44± 3 32 ,P <0 0 5。)结论　铅所致发育神经毒性可能与活性氧自由基 (ROS)Objective To study the neurotoxic mechanisms by lead exposure during developmental period of CNS. Methods The rats in pregnant period and up to the 7th day after delivery were exposed to lead, and their offsprings were affected by lead during pregnant period and breast feeding period. Pb concentration in blood and brain of the offsprings were determined by atomic absorption spectrophotometry. The LPO level, GSH content and SOD activity in brain were also determined. In addition, the number of apoptotic cells in hippocampus and its nearby cortex were counted through in situ labeling method (TUNEL). Results The hippocampus and its nearby cortex in offspring rats exposed to lead, showed the increase of LPO level and apoptotic cells numbers. However, GSH content and SOD activity were decreased. Conclusion The developmental neurotoxicity induced by lead may be associated with the increase of ROS and apoptosis in brain.

关 键 词：铅 新生大鼠 脑氧化损伤 细胞凋亡 发育神经毒性 

分 类 号：R135.11[医药卫生—劳动卫生] R995[医药卫生—公共卫生与预防医学]