雾化吸入白细胞介素-12对小鼠哮喘模型气道炎症和辅助T细胞亚群的影响  

The effects of interleukin-12 inhalation on airway inflammation and T helper cell subsets in mouse asthma models

作　　者：陈欣[1] 林江涛[1] 周童亮[2] 丛笑[2] 王洪超[2] 叶丽亚 李静[2] 乔军[2] 赵武述[2] 

机构地区：[1]中日友好医院呼吸内科,北京100029 [2]中日友好临床医学研究所免疫室

出　　处：《中华内科杂志》2002年第5期313-316,共4页Chinese Journal of Internal Medicine

摘　　要：目的　观察雾化吸入白细胞介素 (IL) 12对哮喘小鼠气道炎症和辅助T细胞 (Th)亚群的影响。方法　将 30只BALB/c小鼠分为 3组 ,每组 10只。哮喘组小鼠采用卵白蛋白 (OVA)腹腔注射致敏加雾化吸入激发的方法制成哮喘模型 ;IL 12干预组小鼠致敏和激发的方法同哮喘组 ,在第 2次致敏的同时雾化吸入IL 12 ;正常对照组小鼠用生理盐水腹腔注射加雾化吸入。OVA激发结束后 2 4h进行支气管肺泡灌洗并收集脾单个核细胞 (SMNCs) ,测定支气管肺泡灌洗液 (BALF)中细胞总数和嗜酸粒细胞计数。采用酶联免疫吸附法测定BALF上清中γ干扰素 (IFN γ)和IL 4水平 ,采用逆转录 聚合酶链反应半定量检测SMNCsIFN γ和IL 4mRNA的表达水平。结果　 (1)哮喘组小鼠BALF中细胞总数和嗜酸粒细胞计数增高 ;IL 12干预组小鼠BALF中细胞总数和嗜酸粒细胞计数减少 ,但与正常对照组相比 ,仍增高。 (2 )哮喘组小鼠BALF上清中IFN γ水平降低 ,IL 4水平增高 ;IL 12干预组小鼠BALF上清中IFN γ水平增高 ,IL 4水平降低 ,但与正常对照组小鼠相比 ,IFN γ水平降低 ,IL 4水平增高。 (3)哮喘组小鼠SMNCsIFN γmRNA表达减弱 ,IL 4mRNA表达增强 ;IL 12干预组小鼠SMNCsIFN γmRNA表达增强 ,IL 4mRNA表达减弱 ,但与正常对照组小鼠相比 ,IFNObjective To observe the effects of aerosol interleukin 12(IL 12) on the antigen induced airway inflammation and T helper cell subsets in mouse asthma models Methods Thirty BALB/c mice were divided into 3 groups,ten in each group The mouse asthma models(asthma group) were established by sensitization and challenge with ovalbumin(OVA) One part of sensitized BALB/c mice(IL 12 group) were interfered with aerosol IL 12 from the beginning of sensitization to the end of challenge The healthy control mice were(control group) treated with normal saline instead of OVA Broncho alveolar lavage fluid (BALF) and spleen mononuclear cells(SMNCs) were collected 24h after the last challenge The total cell numbers and eosinophils(Eos) of BALF were counted The levels of interferon γ(IFN γ) and IL 4 of BALF supernatant were measured by ELISA The mRNA expressions of IFN γ and IL 4 in SMNCs were examined by reverse transcript polymerase chain reaction.Results (1)The total cell counts and Eos counts in asthma group[(12 10±2 23)×10 9/L and (2 02±0 85)×10 9/L,respectively] were significantly increased than those in control group[(6 17±1 34)×10 9/L and (0 05±0 01)×10 9/L ,respectively]( P <0 01),while in IL 12 group the above counts [(8 41±1 71 )×10 9/L and (0 15±0 08)×10 9/L,respectively]were significantly decreased than those of asthma group( P <0 05) (2)The IFN γ and IL 4 levels of BALF supernatant in asthma group [(43 60±15 60)ng/L and (27 15±10 42)ng/L] had significant differences from those in control group[(83 76±19 02)ng/L and (0 84±0 69)ng/L, respectively]( P <0 01) and from those in IL 12 group[(62 30±12 50)ng/L and (6 22±2 28)ng/L, respectively]( P <0 01) (3) The IFN γ and IL 4 mRNA expression of SMNCs in asthma group (0.64±0.14 and 0.89±0.31), respectively) had significant differences from those in control group (1 18±0 34 and 0 71±0 16,respectively)( P <

关 键 词：雾化吸入 白细胞介素12 哮喘 气道炎症 T淋巴细胞亚群 动物模型 

分 类 号：R562.25[医药卫生—呼吸系统] R562[医药卫生—内科学]