甲氧基聚乙二醇修饰脐血淋巴细胞抗原的实验研究  

METHOXY POLYETHYLENE GLYCOL MODIFIED LYMPHOCYTES ANTIGENS IN CORD BLOOD

作　　者：张全[1] 季守平 李素波 熊砚芳[1] 

机构地区：[1]解放军总医院 [2]北京市输血研究所

出　　处：《解放军医学杂志》2002年第6期525-526,共2页Medical Journal of Chinese People's Liberation Army

摘　　要：用甲氧基聚乙二醇(mPEG)修饰脐血单个核细胞 ,并观察修饰后对淋巴细胞表面某些抗原的遮蔽作用及造血干祖细胞体外集落形成能力的影响。流式细胞仪分析结果表明 ,修饰后的脐血淋巴细胞CD3、CD4和CD8抗原与修饰前相比其相对荧光强度明显下降(P <0 0 1) ,剂量越大 ,遮蔽效果越好。当mPEG的浓度达到 12mg/ml时 ,CD3、CD4和CD8表面抗原被遮蔽达 96 %以上。用 3mg/ml、6mg/ml和 12mg/mlmPEG修饰单个核细胞后在体外人干细胞培养基中培养发现 ,形成CFU GM的数量分别为 2 2 83± 14 4 7、2 2 6 7± 14 6 1和2 5 83± 9 99。与对照组 (2 4 0 0±18 4 2 )相比差异无显著性意义 (P >0 0 5 )。提示mPEG修饰脐血淋巴细胞后几乎可以完全遮蔽其表面抗原 ,但不影响干祖细胞在体外的增殖、分化能力。In this research, mononuclear cells from cord blood were modified with 3mg/ml, 6mg/ml and 12mg/ml methoxy polyethylene glycol (mPEG) respectively. The cell surface antigens and the ability of CFU GM proliferation of mPEG modified cord blood lymphocytes were analyzed. Flow cytometric analysis demonstrated that mPEG modified lymphocytes attenuated CD3, CD4 and CD8 antibodies binding to antigens on lymphocyte surface ( P <0 01). Especially in the group of 12mg/ml mPEG, up to 96% antigens were covered. Interestingly, the ability of CFU GM proliferation was not different in mPEG modified cells compared with control cells. These results suggested that mPEGmodification can cover nearly all antigens in the cell surface, whereas it will not change the proliferation and differentiation of cord blood stem cells in vitro .

关 键 词：甲氧基聚乙二醇 脐血 淋巴细胞抗原 实验研究 脐血干祖细胞 MPEG 化学修饰 

分 类 号：R457[医药卫生—治疗学] R392.11[医药卫生—临床医学]