高寒藏药白花假龙胆的组织培养研究  

Studies on tissue culture of Gentianella albifpra

作　　者：霍丽云[1] 郑晓建 

机构地区：[1]山东教育学院生化系,山东济南250013 [2]山东大学生命学院,山东济南250100

出　　处：《中国药学杂志》2002年第6期415-418,共4页Chinese Pharmaceutical Journal

摘　　要：目的　研究不同条件对愈伤组织诱导、继代和分化的影响 ,探讨白花假龙胆的无性快速繁殖的可能性。方法　用白花假龙胆的胚轴、幼叶及未成熟种子诱导愈伤组织并再生植株。结果　选用MS ,B5和N63种培养基 ,其中以附加 2 ,4 D(2 ,4 二氯苯氧乙酸 ) 3.0mg·L-1+KT(激动素 ) 1.0mg·L-1的MS培养基诱导率最高 ;以附加 6 (6 苄基腺嘌呤 )BA 0 .5mg·L-1+NAA(萘乙酸 ) 0 .2mg·L-1的MS培养基分化苗频率最高 ;以附加 2 ,4 D 2 .0mg·L-1+KT 0 .5mg·L-1的MS培养基愈伤组织的生长最好。结论　外植体、培养基、激素、培养条件等对愈伤组织诱导、继代和分化均有明显影响。OBJECTIVE To study the influence of different cultivation conditions on the induction ,maintenance and differentiation of the calli.METHODS Calli and the regenerative plants were induced from young leaves, immature seeds and hypocotyl of gentianella albifora Ma.RESULTS Three kinds of media, MS,B 5 and N 6 were used in the experiment. Calli were induced with the highest frequency onto MS media containing 3 mg·L -1 2,4 D and 1 mg·L -1 KT. The regenerated plants were obtained with the highest frequency on MS medium containing 0.5 mg·L -1 6 BA and 0.2 mg·L -1 NAA.CONCLUSION The induction,maintenance and differentiation of the calli were affected significantly by the basic media and compound of 2,4 D and KT in the media and cultivation conditions.

关 键 词：藏药 白花假龙胆 组织培养 研究 高寒地区 

分 类 号：R282.71[医药卫生—中药学]