单层内皮细胞通透性电导测定方法的建立  

Development of Conductance Measurement Technique for Permeability of Monolayer Endothelial Cells

作　　者：陈蕾[1,2] 王建民[1,2] 金吉成[3] 

机构地区：[1]第三军医大学大坪医院野战外科研究所 [2]重庆大学生物力学与组织工程教育部重点实验室,重庆400044 [3]重庆大学通讯与测控工程学院

出　　处：《生物医学工程学杂志》2002年第2期273-275,283,共4页Journal of Biomedical Engineering

基　　金：教育部访问学者基金资助项目 (教技司 [2 0 0 0 ] 12 3号 )

摘　　要：根据通过溶液的电导受通过面积影响的原理 ,设计了单层内皮通透性的电导测定方法 ,并与白蛋白滤过方法进行了比较。研究结果表明 ,滤膜孔隙间的电导值与滤膜孔隙的总面积有良好相关性 ,应用电导测定方法能反映单层内皮通透性。电导测定方法与白蛋白滤过方法的结合应用 ,能更好地反映内皮单层通透性变化的程度与范围。The conductance measurement method of endothelial permeability has been established by the principle that there is direct ratio between conductance and filter area, and has been compared with the albumin filter method. The results show that there is close correlation between conductance and filter area, the conductance measurement can be used for the detection of the monolayer endothelial cells permeability. The combination of the conductance measurement and the albumin filter measurement method can accurately reflect the permeability change in severity and scope.

关 键 词：单层内皮细胞 通透性 电导测定方法 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]