养胃合剂对环磷酰胺化疗荷瘤小鼠脾淋巴细胞体外增殖反应的影响  

Effect of Yang-wei-mixture on spleen lymphocyte transformation rate of tumor-bearing mice receiving CTX chemotherapy

作　　者：朱涛[1] 郭仲之[2] 沈英森[3] 

机构地区：[1]暨南大学医学院第一附属医院,广州510630 [2]广州中医药大学第一附属医院,广州510407 [3]暨南大学医学院,广州510632

出　　处：《广东医学》2002年第6期566-568,共3页Guangdong Medical Journal

基　　金：广东省中医药管理局科研资助项目 (编号 :9840 7)

摘　　要：目的　观察养胃合剂对环磷酰胺化疗荷瘤小鼠脾淋巴细胞体外增殖反应的影响。方法　对C57BL小鼠进行肉瘤 180 (S180 )接种造模 ,给予养胃合剂灌胃和环磷酰胺 (CTX)腹腔注射 ,停药 2 4h后 ,将各组小鼠断颈处死 ,无菌取脾 ,进行体外试验 ,采用MTT法测定小鼠脾淋巴细胞转化率。结果　用高、低剂量养胃合剂的荷瘤化疗小鼠脾淋巴细胞转化率 ,均显著高于荷瘤单纯化疗组 (P <0 0 1)。结论　养胃合剂能促进环磷酰胺化疗荷瘤小鼠脾淋巴细胞体外增殖反应 ,改善细胞免疫功能 ,发挥其减毒作用。Objective To observe the effect of Yang-wei-mixture(YWM) on spleen lymphocyte transformation rate of tumor-bearing mice receiving chemotherapy. Methods S 180 -bearing mice were treated with CTX and YWM and were killed 24 hours after discontinuation of medication. Spleens of mice were obtained for testing spleen lymphocyte transformation rate by MTT assay in vitro. Results Spleen lymphocyte transformation rates of both chemotherapy groups treated with high or low dosage of YWM were greater than that treated with chemotherapy alone(P<001). Conclusion YWM can increase spleen lymphocyte transformation rate and improve immunity of tumor-bearing mice receiving CTX chemotherapy.

关 键 词：养胃合剂 环磷酰胺 化疗 荷瘤小鼠 体外增殖反应 影响 脾淋巴细胞 

分 类 号：R285.5[医药卫生—中药学] R273.05[医药卫生—中医学]