应用基因组原位杂交鉴定杂交后代中的簇毛麦染色体  

Identificaion of Haynaldia villosa Chromosome in Hybrids by Genomic in Situ Hybridization

作　　者：张小萍[1] 

机构地区：[1]西南师范大学生命科学学院,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2002年第3期401-403,共3页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：西南师范大学自然科学基金资助

摘　　要：用地高辛 (Digoxigenin 11 dTup)标记的簇毛麦染色体组DNA为探针 ,以普通小麦“中国春”总DNA作封阻进行基因组荧光原位杂交 ,对小麦与簇毛麦杂交和回交后代进行检测 .结果显示 ,在杂交后代的 2 8条染色体中有 7条簇毛麦染色体 ,在发生部分染色体加倍的回交后代中鉴定出含 7条簇毛麦染色体的易位系 .Detection of the haynaldia villosa chromosomes in hybrids between common wheat and haynaldia villosa was undertaken using genomic fluorescent in situ hybridization(GISH). Digoxigenin-11-dTup labeled total genomic DNA of haynaldia villosa was used as the probe and the unabelled total DNA of commom wheat Chinese Spring(CS) as blocking DNA. The results from GISH revealed that there are 7 haynaldia villosa chromosomes in F 1 hybrids and backcross plants, translocation has taken place between one wheat chromosome and one H.villosa chrimosome.In one backcross plant, in which some wheat chromosomes reduplicated. These materials will be useful in transferring the valuable genes in H.villosa to wheat

关 键 词：鉴定 杂交后代 染色体 基因组原位杂交 小麦 簇毛麦 易位系 属间杂交 

分 类 号：S512.1[农业科学—作物学] Q343.2[生物学—遗传学]