葱莲凝集素基因的克隆(英文)  

Molecular Cloning of Lectin Gene from Zephyranthes Candida

作　　者：庞永珍[1] 姚剑虹[1] 申国安[1] 唐克轩[1] 谈锋[2] 

机构地区：[1]复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室,上海200433 [2]西南师范大学生命科学学院,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2002年第3期433-436,共4页Journal of Southwest China Normal University(Natural Science Edition)

摘　　要：利用RACE -PCR技术从葱莲叶片中克隆出葱莲凝集素的全长cDNA .通过比较葱莲同其它石蒜科植物的凝集素基因序列和推测的氨基酸序列 ,发现葱莲凝集素基因编码一具有信号肽的前体蛋白 .葱莲凝集素同其它石蒜科植物凝集素一样为具有The full length cDNA of Zephyranthes candida lectin gene was cloned from leaves by using RACE PCR. Through comparative analysis of Zephyranthes candida lectin gene and its deduced amino acid sequence with those of other Amaryllidaceae species, Zephyranthes candida lectin gene was found to encode a precursor lectin with signal peptide. Zephyranthes candida lectin was a mannose binding lectin with three mannose binding boxes like lectins from other species of Amaryllidaceae.

关 键 词：CDNA 凝集素基因 RACE-PCR 葱莲 基因克隆 石蔚料 氨基酸序列 基因编码 

分 类 号：Q949.718.2[生物学—植物学] Q785