太湖水华微囊藻毒素对小鼠免疫功能的影响  

Effects of Tai lake bloom microcystins on immune function of mice

作　　者：孙露[1] 沈萍萍[1] 周莹[1] 华子春[1] 

机构地区：[1]南京大学生物化学系医药生物技术国家重点实验室,江苏南京210093

出　　处：《中国药理学与毒理学杂志》2002年第3期226-230,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金资助项目 (2 0 0 770 12 )~~

摘　　要：目的　蓝藻水华已成为太湖的严重污染物 ,本文旨在阐明其对哺乳动物的潜在危害。方法　应用巨噬细胞吞噬实验、脾淋巴细胞转化法、溶血分光光度法、反转录 聚合酶链反应以及酶联免疫测定等手段较系统地分析了太湖微囊藻毒素对小鼠免疫功能的影响。结果　当ip 5mL·kg- 1(相当于 2 0mg·kg- 1)连续 7d时 ,该微囊藻毒素不仅引起小鼠肝、脾肿大 ,腹腔巨噬细胞吞噬功能降低 ,而且抑制小鼠的T淋巴细胞增殖及B淋巴细胞产生抗体的能力 ,使得肿瘤坏死因子αmRNA的表达及其在血清中的含量明显降低。结论　太湖微囊藻毒素对小鼠的毒性涉及到免疫系统基因和分子的多层次、多方面 ,它对小鼠免疫功能产生了明显的抑制作用。AIM In order to investigate the latent risk of Tai lake cyanobacteria blooms on mammals. METHODS The mice were administered intraperitoneally with the microcystins extract from cyanobacteria blooms collected from the Tai lake in dose of 5 mL·kg -1 of body weight (equal to 20 mg·kg -1 of body weight), over a period of 7 d. On d 8, the immune function of mice was measured with macrophagocyte phagocytosis assay,the splenocyte proliferation assay, hemolytic spectrophotometry, reverse transcription polymerase chain reaction(RT PCR) and enzyme labeled immunosorbent assay(ELISA). RESULTS Toxicity was displayed by a drop in body weight, increase in spleen index, reduction of phagocytic activity of phagocyte. The mitogenic response of splenic T cell to concanavalin A and the primary antibody response to sheep erythrocyte were significantly decreased in the BMC treated mice. The results of PCR and ELISA elucidated that BMC down regulated the expression level of tumor necrosis factor(TNF) α mRNA and decreased the content of TNF α in mouse serum. CONCLUSION The findings indicate that BMC obviously mediated the immunosuppression on mice.

关 键 词：太湖 水华 微囊藻毒素 小鼠 免疫功能 影响 

分 类 号：R99[医药卫生—毒理学]