人锰超氧化物歧化酶的紫外可见吸收光谱研究  

Investigation of UV-Visible Absorp tion Spectra of Recombinant Human Manganese Superoxide Dismuta se

作　　者：李连之[1] 欧阳砥[2] 邱晓航[2] 胡绪英[2] 周永洽[2] 申泮文[2] 

机构地区：[1]聊城师范学院化学系,聊城252059 [2]南开大学化学系,天津300071

出　　处：《无机化学学报》2002年第7期688-692,共5页Chinese Journal of Inorganic Chemistry

摘　　要：研究了重组人锰超氧化物歧化酶(MnSOD)的紫外可见吸收光谱。经光学电负性和摩尔吸收系数分析,重新归属了酶中Mn?的可见光谱,首次报道了酶中Mn?的电荷转移光谱。研究表明,MnSOD中显示的电荷转移谱带全部是由His咪唑基π轨道向金属轨道的LMCT跃迁。The UV-visible absorption spectra o f recombinant human manganese superoxide dismutase(MnSOD)were investigated.The visible absorpti on spectrum of Mn ?was assigned anew and the charge tran sfer spectrum of Mn ?in MnSOD was first reported accordin g to the optical electronegativity a nd mole absorption coefficient of Mn SOD.These results have shown that all the charge transfer spectra displayed i n MnSOD are attributed to the ligand metal charge transfer spectra(LMCT)from theπorbitals of imidazole of histidine t o metal orbitals.

关 键 词：锰超氧化物歧化酶 紫外可见光谱 电荷转移光谱 光学电负性 

分 类 号：Q554.9[生物学—生物化学]