富含血小板血浆制备过程中血小板CD62p表达  

CD62p Expression in Platelet during the Preparation Course of Cryopre-servated Platelet-Rich Plasma

作　　者：刘景汉[1] 欧阳锡林[1] 石群[1] 骆群[1] 李锡金[1] 王海宝[1] 陈民才[1] 韩玮[1] Dayong Gao 

机构地区：[1]解放军总医院输血科,解放军临床输血中心,北京,100853 [2]University of Kentucky Lexington, KY40506 USA

出　　处：《中国实验血液学杂志》2002年第3期253-256,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目 编号 3 9970 812

摘　　要：为了探索大批量制备冰冻保存富含血小板血浆 (Cryo PRP)全过程中影响血小板质量变化的因素 ,以优化Cryo PRP制作过程 ,提高Cryo PRP质量 ,采用流式细胞术测定Cryo PRP全过程中血小板膜表面CD6 2 p表达。冰冻保存血小板全过程包括血液采集、离心制备、添加DMSO、- 80℃冰箱保存和 38℃水浴解冻。结果表明 ,在血液采集、离心制备、添加DMSO过程中血小板膜表面CD6 2 p表达逐步升高 ,但升高幅度较小 ,血小板CD6 2 p阳性率约为 5 .2 5 % ,而降温、解冻过程中血小板CD6 2 p阳性率有一个突然的升高 ,其幅度约占整个过程的 82 % ,对血小板的损害较大。结论 :在Cryo PRP的制备过程中 ,血小板的离心制备和DMSO的添加方式均可以得到良好的优化 ,而对冰冻富含血小板血浆的质量影响也易于控制 ,但冰冻保存对血小板的损害较大 ,且不可避免。因此 ,迫切需要一种新型的血小板冰冻保护液和改进冰冻保存方法 ,以减轻冰冻损害 。In order to explored the factors that affect CD62p expression in platelet during the whole course of cryopreservated platelets preparation, CD62p expression of platelet was evaluated by flow cytometry assay. The whole course of cryopreservated platelets preparation in order included whole blood collection, centrifugation for fresh platelet rich plasma preparation, addition of dimethyl sulfoxide, and freeze in -80℃ refrigeratory and thaw in 38℃ water bath. Result showed that the CD62p expressed slowly from whole blood collection to addition of dimethyl sulfoxide, but expressed abruptly after freeze and thaw and it occupied 82 per cent of the whole expression. It was concluded that the whole blood collection, centrifugation for fresh platelet riched plasma preparation and addition of dimethyl sulfoxide were optimized in the whole course, but the damage to platelets in the whole course of cryopreservation could not be avoided. It suggests that improved techniques are needed to reduce the damage to cryopreservated platelets.

关 键 词：富含血小板血浆 血小板冰冻保存 CD62P 流式细胞术 

分 类 号：R457.1[医药卫生—治疗学]