利用基因芯片对一高保守基因进行肿瘤相关分析  

Tumor Relevance Analysis of A Highly Conserved Gene by Using Gene Microarray Hybridization

作　　者：程超[1] 周宗祥[1] 徐明[1] 赵炜[1] 徐坚[1] 曾立[1] 黄燕[1] 吴奇涵[1] 戴建锋[1] 应康[1] 谢毅[1] 毛裕民[1] 

机构地区：[1]复旦大学生命科学院遗传学研究所,上海200433

出　　处：《遗传》2002年第3期227-231,共5页Hereditas（Beijing)

基　　金：国家自然科学基金 ( 30 0 70 80 6 )

摘　　要：本文对大规模人类cDNA测序过程中获得的一条高保守基因进行了初步功能研究 ,生物信息学研究发现该基因在人类、小鼠、果蝇、拟南芥和裂殖酵母中都有很高的保守性 ,其他分析预测该基因可能具有肿瘤相关性。RT -PCR分析表明 ,该基因在成人和胎儿组织中广谱表达。利用基因芯片分析该基因在 7例肝癌、5例胰腺癌、2例喉癌和 2例肺癌中表达情况 ,结果证实了该基因的肿瘤相关性 ,并且提示该基因在不同肿瘤类型中可能处于不同的地位。Preliminary function research of a highly conserved human gene,which was cloned from human fetal cDNA library during large-scale cDNA sequencing,is illustrated in this article.Bioinformatics analysis indicates that this gene is highly conserved in human?mouse?fruit fly?thaliana and fission yeast.Other bioinformatics analysis implies its relevance with tumors.RT-PCR analysis shows its wide-ranging expression patterns.Its expression in 16 cancer cases(including 7 liver cancer cases?5 pancreas cancer cases?2 larynx cancer cases and 2 lung cancer cases) is studied by using gene microarray analysis.The result shows its relevance with tumors and implies it may have different status in different classification of tumors.

关 键 词：基因芯片 高保守基因 肿瘤相关分析 反转录-PCR 结构域 

分 类 号：Q789[生物学—分子生物学]