反义hTR抑制人胰腺癌P3细胞系端粒酶活性和增殖的研究  

Inhibition Effects of Phosphorothioate-Modified Antisense hTR on the Telomerase Activity and the Growth of P3 Cells Derived from Pancreatic Cancer in Culture

作　　者：张红梅[1] 张丽珊[1] 杨德同[2] 周家华[2] 洪泽辉[1] 

机构地区：[1]东南大学医学院遗传中心,南京210009 [2]东南大学附属中大医院胆胰外科,南京210009

出　　处：《遗传》2002年第3期237-241,共5页Hereditas（Beijing)

基　　金：江苏省科委社会发展课题资助 ( 98-BJ80 )

摘　　要：设计针对端粒酶RNA模板序列并经硫代磷酸修饰的正义、反义和随机序列寡核苷酸 ,以正常人成纤维细胞作对照 ,观察其对人胰腺癌细胞端粒酶活性和细胞生长增殖的影响作用以及对正常细胞是否有毒副作用。结果表明 :针对hTR模板区的反义寡核苷酸能降低胰腺癌P3细胞端粒酶活性 ,抑制细胞生长 ,促进细胞周期改变并诱导细胞发生凋亡 ,而且对正常细胞没有明显的毒副作用。因此我们认为利用反义技术封闭hTR基因很可能成为治疗肿瘤的安全、有效手段之一。This paper is to investigate PS ODN's (antisense PS ODN of hTR,sense PS ODN of hTR and random sequence) effects on telomerase activity and proliferation of P3 pancreatic cancer cells,and to find a novel method for gene therapy of pancreatic cancer.The results indicate that the anti hTR complementary to the template region of hTR is sufficient to inhibit P3 cell telomerase activity and cell proliferation in vitro,and as a result,they can lead to a profound induction of programmed cell death.Telomerase represents an interesting and promising anticancer drug target and antitelomerase technology may have potential significance in tumor therapy.

关 键 词：反义HTR 抑制 人胰腺癌P3细胞系 端粒酶活性 细胞周期 细胞增殖 

分 类 号：Q75[生物学—分子生物学]