17种金线鲃核DNA含量及倍性的研究  

Nuclear DNA Content and Ploidy of Seventeen Species of Fishes in Sinocyclocheilus

作　　者：肖蘅[1] 张仁东 冯建国 欧阳明[2] 李维贤[3] 陈善元 昝瑞光 

机构地区：[1]云南大学生命科学与化学学院,云南昆明650091 [2]云南大学分析测试中心,云南昆明650091 [3]云南省石林县黑龙潭水库,云南石林652200

出　　处：《Zoological Research》2002年第3期195-199,共5页动物学研究（英文）

基　　金：~~

摘　　要：采用血涂片、Feulgen染色和显微分光光度技术 ,测定了金线属 (Sinocyclocheilus) 17个种的核DNA含量。结果显示 ,除侧条金线 (S .lateristritus)中采自云南沾益炎方山那边的样本之 2C值为 7 79pg外 ,其余的核DNA 2C值都集中分布在 4 19～ 4 86pg范围 ,大体与其近缘四倍体种 2C值相同或相近 ,是其近缘二倍体种 2C值的大约 2倍。据此我们推断 ,这 17种金线很可能都是四倍体 ,个别种还含有八倍体的类型 ,金线属可能是整属的四倍体起源。The genus Sinocyclocheilus (Cypriniformes: Cyprinidae: Barbinae) is endemic to China and only found in Yunnan, Guizhou and Guangxi Provinces. The nuclear DNA content of two species and the ploidy level of three species in Sinocyclocheilus had been determined in the middle of 1980s. In recent years, more and more new species were reported in this genus. Therefore, it is necessary to analyze the nuclear DNA content, ploidy level and evolution of the species in the genus. In the present paper, by means of the red cell spreading-Feulgen staining-cytophotometry technique, we have measured the nuclear DNA content of 17 species in Sinocyclocheilus from Yunnan, Guizhou and Guangxi Provinces and distributed in the drainage of Nanpanjiang, Jinshajiang, Beipanjing, Yuanjiang, Hongshuihe and Youjiang rivers. The results showed that the nuclear DNA content of all 17 species were between 4 19 and 4 86?pg, as the same level of the related tetraploid species, doubled that of the related diploid species. It is likely that all of these 17 species and the whole genus Sinocyclocheilus have tetraploid origin.

关 键 词：金线BA 核DNA含量 倍性 

分 类 号：Q959.4[生物学—动物学]