用cDNA微阵列研究内毒素休克小鼠肺组织基因表达谱的改变  

A Study on The Changes of Gene Expression Profiles in Lung Tissues During Endotoxic Shock Using cDNA Miroarray

作　　者：袁开宇[1] 赵震宇[1] 刘瑛[1] 邹江[1] 刘梅冬[1] 陈广文[1] 尢家騄[1] 肖献忠[1] 

机构地区：[1]中南大学湘雅医学院病理生理学教研室,长沙410078

出　　处：《生物化学与生物物理进展》2002年第3期402-406,共5页Progress In Biochemistry and Biophysics

摘　　要：利用cDNA微阵列检测了小鼠内毒素休克 2h及 2 0h肺组织基因表达谱的改变 .发现内毒素休克 2h有12 8个基因表达上调 ,3个基因下调 ;内毒素休克 2 0h有 5 1个基因表达上调 ,2 1个下调 .并用RT PCR进一步验证了结果的可靠性 .初步分析了基因表达谱改变的意义 .The gene expression profiles of 1 176 genes were analyzed using Atlas TM Mouse cDNA array 1 2 in Babl/c mice lung tissues during endotoxic shock. Results showed that the expression of 128 genes was up regulated and 3 genes down regulated at 2 hours after exposure to endotoxin; the expression of 51 genes was up regulated and 21 genes down regulated at 20 hours after exposure to endotoxin. The changes in gene expression were further confirmed by RT PCR. The significance of above changes in gene expression was analyzed. The results would be helpful to elucidating the molecular mechanism of endotoxic shock.

关 键 词：CDNA微阵列 内毒素休克 小鼠 肺组织 基因表达谱 改变 

分 类 号：R631.4[医药卫生—外科学]