随机扩增多态性DNA技术对结核分支杆菌菌株鉴别及其耐药相关性分析  

Strains Differentiation and Analysis on Resistant Relation of Mycobacterium Tuberculosis by Random Amplified Polymorphism DNA

作　　者：王晓樱[1] 鲍朗[1] 谢勇恩[1] 张万江[1] 陈玮[1] 刘益民 谢益新 罗涛 

机构地区：[1]四川大学华西基础医学院感染免疫研究室,中国四川成都610041 [2]成都市结核病医院,中国四川成都610015

出　　处：《生命科学研究》2002年第2期171-174,共4页Life Science Research

基　　金：教育部博士点基金资助项目 ( 2 0 0 0 0 6 30 0 6 )

摘　　要：用引物G1和L1扩增的 16S～ 2 3SrDNA间隔区作为模板DNA ,再用引物AP5 0进行随机扩增多态性DNA(RandomAmplifiedPolymorphicDNA ,RAPD)分析 .经AP5 0扩增出的各菌株DNA片段 ,用 1.2 %的琼脂糖凝胶电泳EB染色 ,可获得 1或 2个大小不同的DNA片段 (390～ 10 31bp) ,各株间差异明显 .结果表明 ,不同菌株有不同的DNA多态性 ,药物敏感株与耐药株之间多态性差异明显 ,耐药株中耐药谱相似或相同的菌株间其多态性也相似 ,RAPD有助于简便、快速、有效地了解菌株间的克隆相关性及耐药性传播 .Amplification of the region separating the genes coding for 16?S and 23?S rDNA was performed with primers G1 and L1. Those PCR products were then used as the target DNA for random amplified polymorphism DNA (RAPD) analysis with arbitrary primer AP50. After RAPD with AP50, one or two DNA fragments with different sizes (390 to 1?031 bp) were separated using electrophoresis with EB stain from each strain. The difference is significant among strains. So it can be concluded that different strains had different DNA polymorphism, which are significant between drug-sensitive and drug-resistant strains. Drug-resistant strains with similar or common antibiotic profile had similar DNA polymorphism. Thus, RAPD is helpful for the simple, rapid and efficient investigation of colonal relation among strains and spread of antibiotic resistance.

关 键 词：随机扩增多态性DNA技术 结核分支杆菌 菌株鉴别 耐药相关性 

分 类 号：R378.911[医药卫生—病原生物学]