早期心肌梗死后诊断若干免疫组织化学指标的敏感性比较  

COMPARISON OF SENSITIVITY BETWEEN SEVERAL IMMUNOHISTOCHEMICAL MARKERS FOR THE POSTMORTEM DIAGNOSIS OF EARLY MYOCARDIAL INFARCTION

作　　者：胡丙杰[1] 程海鹰 陈玉川[1] 何靖 祝家镇[1] 毕启明[1] 刘于武 

机构地区：[1]中山医科大学法医病理学教研室,广州510080 [2]广东省增城市公安局,增城511300

出　　处：《中国组织化学与细胞化学杂志》2001年第4期406-411,473,共7页Chinese Journal of Histochemistry and Cytochemistry

基　　金：卫生部科研基金资助项目 (No.96 - 1- 12 7);广东省自然科学基金资助项目 (No.980 0 78);卫生部优秀青年科技人才专项基金资助项目 (No.970 0 12 );广东省医药卫生青年科研基金资助项目 (No.B19980 13);中山医科大学"2 11工程"基金资助项目

摘　　要：为探讨纤维连接蛋白 (Fn)、纤维蛋白原 (Fg)、补体 (C5)、肌红蛋白 (Mb)、肌动蛋白 (HHF35 )、结蛋白(Dm)六种指标在诊断早期心肌梗死的敏感性 ,建立大鼠急性心肌缺血模型 ,应用免疫组织化学方法和图像分析与统计学处理系统 ,检测缺血心肌细胞内 Mb、 HHF35、 Dm的缺失面积和 Fn、 Fg、 C5的阳性反应面积。结果 :Mb、 HHF35、 Dm在大鼠急性心肌缺血 15 m in即可出现缺失 ,Fg、C5在心肌缺血 15 min即可出现阳性反应 ,而 Fn直到心肌缺血 3h才出现阳性反应。随缺血时间延长 ,Dm、 HHF35、 Mb的缺失面积和 Fg、 C5、 Fn的阳性反应面积逐步增大。表明 Dm、 HHF35、 Mb、Fg、 C5是反映早期心肌梗死的灵敏的形态学指标 。To investigate the sensitivity of fibronectin(Fn), fibrinogen(Fg), complement(C 5), myoglobin(Mb), actin(HHF35) and desmin(Dm) for the postmortem diagnosis of early myocardial infarction, the myocardial ischemia model was established in rats. The areas of Mb, HHF35 and Dm depletion,and the areas of Fn, Fg and C 5 positive reaction in the ischemic myocardial cells were studied with immunohistochemistry, image analysis technique and statistical system. The results showed that the depletion of Dm, HHF35 and Mb as well as the positive staining of Fg and C 5 in ischemic myocardial cells appeared at 15 min after myocardial ischemia, but the positive staining of Fn did not occur until 3 hours after the ischemia. With the lapse of time, the areas of Dm, HHF35, Mb depletion and Fg, C 5 Fn positive staining gradually enlarged. It is suggested that Mb, HHF35, Dm, Fg and C 5 are more sensitive markers than Fn for the postmortem diagnosis of myocardial infarction.

关 键 词：早期心肌梗死 诊断 敏感性 免疫组织化学指标 

分 类 号：R542.22[医药卫生—心血管疾病]