DNA氧化损伤的免疫组织化学研究(英文)  

IMMUNOHISTOCHEMICAL STUDY OF DNA OXIDATIVE DAMAGE

作　　者：冯仁田[1] 郝志勇[1,2] 谭会兵[1,2] 何维[1,2] Hirotom o Ochi 

机构地区：[1]中国药物生物制品检定所生化药品室 [2]北京,100050 [3]日本老化制御研究所

出　　处：《中国组织化学与细胞化学杂志》2001年第2期224-227,T005,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：日本老化制御研究所资助课题!(No.Ja ICA1997- 2 0 0 0 )

摘　　要：采用目前公认的 DN A氧化损伤标志物 8- OHd G的单抗 ,利用 L SAB法研究不同年龄组 BAL B/ C小鼠胸腺和脾脏8- OHd G的生成水平 ,对免疫组织在衰老过程中 DNA氧化损伤的水平进行免疫组织化学研究 ,以探讨免疫系统的自由基损伤对衰老的影响。结果表明 ,在胸腺 ,8- OHd G+细胞密度随增龄而增多 ,并主要位于髓质区 ;脾脏中的阳性细胞则无明显的增龄性变化 ,但胞染形态却存在明显差异。本研究结果显示 ,衰老过程中 ,免疫细胞内 8- OHd G含量发生改变 ,免疫系统受到了氧自由基的损伤。 8- OH dObjective\ To evaluate the levels of 8 Hydroxy 2' deoxyguanosine (8 OHdG) in the lymphoid organs of different aged BALB/c mice.\;Methods\ An LSAB immunohistochemical method was used to detect the formation of 8 OHdG in the thymus and spleen of 1 , 5 and 10 month of BALB/c mice.\;Results\ The results showed that there was more extensive staining in the nuclei of thymic cells in the elder mice, and the 8 OHdG formation was mostly situated in medulla of thymic tissues. From cortex to medulla, positive cells were increased in number. In splenic tissues, the nucleus stainings were predominately localized in red pulp, but no visible age related changes were observed both in the number and the density of the 8 OHdG + spleen cells. In addition, the shapes of nucleus stainings appeared not the same among these spleen tissues with different ages.\;Conclusions\ The present results indicate that immune system may suffer from oxidative damage of endogenous and extrinsic oxygen free radicals in older mice, which could be reflected by the presence of 8 OHdG within immunocytes. Therefore, 8 OHdG could be a biomarker for DNA oxidative damage in immunosenescence.\;

关 键 词：衰老 8-OHDG 自由基损伤 DNA氧化损伤 免疫组织化学 

分 类 号：R339.38[医药卫生—人体生理学] Q419[医药卫生—基础医学]